Lymphocyte subsets, T, B, NK cell counts
General Information
- Lab Name
- Lymphocyte subsets, T, B, NK cell counts
- Lab Code
- TCSNK
- Epic Ordering
- T Cell Subsets w/NK Cells
- Description
This assay provides absolute cell counts and percentages for CD4, CD8, CD3 T cell subsets, CD19 B cell subsets, and NK cells.
All lymphocyte subset panels offered:
T cell subsets CD4/CD8 only, counts [TCS48] - CD4, CD8
T cell subsets CD3/CD4/CD8, counts [TCSA] - CD4, CD8, CD3
Lymphocyte subsets, T and B cell counts [TCSP] - CD4, CD8, CD3, CD19
Lymphocyte subsets, T, B, NK cell counts [TCSNK] - CD4, CD8, CD3, CD19, CD56
CD19+ B Cell Count [BCS19] - CD19 only- References
https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.21360
- Synonyms
- CD4, CD8, NK, T Cell Subsets plus Natural Killer Cells
- Components
-
Code Name CD4P % CD4 CD4A Abs CD4 Lymphocyte Cnt CD8P % CD8 CD8A Abs CD8 Lymphocyte Cnt CD19P % CD19 CD19A Abs CD19 Lymphocyte Count 3NNKPP % CD3_, CD16+56+ 3NNKPA Abs CD3_CD16+/56+ Lymph Count CD4CD8 CD4/CD8 Ratio CD3P % CD3 CD3A Abs CD3 Lymphocyte Cnt NFDA4 Test Comment
Interpretation
- Method
This is a single platform lymphocyte subset assay and has been approved by the U.S. Food and Drug Administration.
- Reference Range
- See individual components
- Ref. Range Notes
%CD4 [33-61] Abs CD4 [0.730-2.250] Thou/ul %CD8 [14-35] Abs CD8 [0.250-1.240] Thou/ul CD4/CD8 [1.00-3.78] %CD3 [57-87] Abs CD3 [1.040-3.400] Thou/ul %CD19 [9-23] Abs CD19 [0.160-0.390] Thou/ul %NK [2-27] Abs NK [0.027-0.693] - Interferences and Limitations
The stability limit for this FDA approved assay is 24 hours
- References
https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.21360
- Guidelines
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 or 5 mL Lavender (EDTA) tube
Unacceptable: GEL SEPARATOR tubes - Handling Instructions
Specimens must be tested within 24 hours of collection for this FDA assay. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Specimens must be transported at room temperature. Refrigerated or frozen specimens will be canceled and a re-draw will be requested.
- Quantity
-
requested: 3-4 ml
minimum: 1 ml
Processing
- Processing
Stability: 24 hours @Room Temperature
SPS: Sample can be shared with CBC. Do not add on test if sample will be received in the Hematopathology lab outside the 24-hour stability limit. If sample is approaching 24 hour stability limit, contact hematopathology lab (206-606-7060) to notify them that a specimen is coming and send immediately.
Outside Laboratories: Specimens must be tested within 24 hours of collection. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Contact Lab Med Call Center 206-685-6066 to discuss transportation options. Specimens can be sent directly to the hematopathology lab as well (include contact info).
Performance
- LIS Dept Code
- Hematopathology (HP)
- Performing Location(s)
-
Other Hematopathology
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology hours:
5:30 am - 6:30 pm Monday through Saturday
7:30 am - 4:00 pm Sunday, and with reduced staffing on major holidays - Frequency
- Monday-Sunday.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 86355, 86357, 86359, 86360
- LOINC
- 18768-2
- Interfaced Order Code
- UOW495