Specialty testing for Molecular Microbiology

Founded in 2001, the UW Molecular Microbiology laboratory provides real-time PCR and DNA sequence-based testing for hospitals and clinics from all over the world. Our approaches provide highly accurate, rapid and sensitive capture of genomic information which, when integrated with the traditional discipline of clinical microbiology, enables robust diagnoses and provides accurate descriptions of new and emerging microbial pathogens. The molecular microbiology division offers a wide range of testing for detection and identification of specific microorganisms and gene products, as well as microbial DNA directly from clinical samples.

More information regarding submission of samples can be obtained viewing individual test pages below.

Direct detection of microbial DNA from tissues

DNA is isolated from specimen and amplified by conventional PCR using a battery of broad-range primers dependent on the test type requested. Amplified products are sequenced and the organism(s) identified on the basis of sequence data. Select a test type below to learn more.

Detection and identification of specific pathogens

DNA is isolated from a cultured organism or direct patient specimen and amplified using primers developed specifically to detect a particular organism. Amplifications are performed either as conventional or real-time PCR. Select a test type below to learn more.

Identification of cultured organisms by DNA sequence based methods

DNA of the cultured organism is isolated and amplified using broad-range primers and conventional PCR conditions. Amplified products are then sequenced and the organism is identified on the basis of the sequence data. Select a test type below to learn more.

Specific gene detection in cultured organisms

DNA of a cultured organism is isolated and amplified using primers developed specifically to detect a particular gene. Amplifications are performed under real-time PCR conditions and results are based on the presence or absence of amplification. Select a test type below to learn more.

Organism typing

References

Associated Tests

Code Name Specimen
ASEQID AFB ID by Sequence Analysis Pure Culture
ASPDNA Aspergillus fumigatus DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
BSEQID Bacteria ID by Sequence Analysis Pure Culture
NGS16S Bacterial detection by 16S NGS This only available as a reflexive test,...
NRBDNA Bacterial DNA Detection by PCR (without reflex to NGS) Tissue (Fresh frozen or paraffin-embedde...
BWGSID Bacterial ID by Whole Genome Sequencing Pure Culture
BCTDNA Bacterial PCR reflex NGS Tissue (Fresh frozen or paraffin-embedde...
NGSTYP Bacterial Strain Typing by Whole Genome Sequencing Pure culture of bacterial organisms
BRTDNA Bartonella DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
COCDNA Coccidioides DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
CRYDNA Cryptococcus DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
ENBDNA Enterobacterales DNA Detection This only available as a reflexive test,...
NGSITS Fungal DNA Detection by ITS Next Gen Seq This only available as a reflexive test,...
NRFDNA Fungal DNA Detection by PCR (without reflex to NGS) Tissue (Fresh frozen or paraffin-embedde...
FUNDNA Fungal PCR reflex NGS Tissue (Fresh frozen or paraffin-embedde...
HISDNA Histoplasma DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
LEGDNA Legionella DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
LSHDNA Leishmania species DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
MECPCR mecA by PCR Pure culture
MSEQID Mold ID by Sequence Analysis Pure Culture
MTBWGR MTB Resistance by Whole Genome Sequencing Pure Culture
TBCDNA M. tuberculosis complex DNA Detection Tissue (Fresh frozen or paraffin-embedde...
MAVDNA Mycobacterium avium complex DNA Detection Tissue (Fresh frozen or paraffin-embedde...
MCHDNA Mycobacterium chimaera DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
MPLDNA Mycoplasma and Ureaplasma DNA Detection by PCR Tissue (Fresh frozen or paraffin-embedde...
NTMDNA Nontuberculous Mycobacteria DNA Detection Tissue (Fresh frozen or paraffin-embedde...
PNEDNA Pneumocystis DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
TOXDNA Toxoplasma DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
TPLDNA Treponema pallidum DNA detection by NAAT Tissue (Fresh frozen or paraffin-embedde...
TPLSWB Treponema Pallidum DNA detection, Mucocutaneous UTM Swab
TWHDNA Tropheryma whipplei DNA detection by PCR Tissue (Fresh frozen or paraffin-embedde...
YSEQID Yeast ID by Sequence Analysis Pure Culture

Last updated 2024-11-19T21:15:13.874670+00:00