Fungal DNA Detection by ITS Next Gen Seq

General Information

Lab Name

Fungal DNA Detection by ITS Next Gen Seq

Lab Code

NGSITS

Epic Name

(not orderable in Epic)

Description

Fungal ITS Next Generation Sequencing is only available as reflexive test from Fungal PCR reflex NGS [FUNDNA] when there is evidence of multiple fungal templates present.

Results in 10 business days after reflex from Fungal PCR reflex NGS [FUNDNA]

Broad range PCR and Sanger sequencing can be used to perform accurate genus- or species-level taxonomic assignment for several fungal gene targets. Unfortunately, when PCR amplification is performed on polymicrobial specimens, the presence of multiple templates causes interference during Sanger sequencing such that results are generally uninterpretable. In contrast, next-generation sequencing methods provide individual sequence data for large numbers of DNA templates, allowing each to be classified independently. This permits fungal species identification for samples from which usable data cannot be obtained by conventional clinical methods.

Synonyms

broad range fungal PCR, broad range fungi PCR, fungal identification, fungal ITS, Fungal PCR, fungal sequencing, Fungi PCR, fungi sequencing, ITS deep sequencing, ITS PCR, ITS sequencing, molecular fungal, molecular fungi, Next Generation Sequencing, NGS, polymicrobial sequencing, universal fungal PCR, universal fungi PCR

Components

Code	Name
NGITSI	Fungal ITS Gene Next Gen Seq Specimen
NGITRS	Fungal ITS Gene Next Gen Seq Results
NGITIN	Fungal ITS Gene Next Gen Seq Interpretation
NGITSR	Fungal ITS Gene Next Gen Seq Requests
NGITME	Fungal ITS Gene Next Gen Seq Method Note