Enterobacterales DNA Detection

General Information

Lab Name

Lab Code

ENBDNA

Epic Ordering

Enterobacterales DNA Detection

Description

Enterobacterales DNA Detection by PCR is only available as a reflexive test when results indicate the presence of bacteria in the Order Enterobacterales from Bacterial DNA Detection by PCR (BCTDNA) or Bacterial detection by 16S NGS (NGS16S).

Results in 2-3 business days after reflex.

The family Enterobacteriaceae (within the order Enterobacterales) contains over 60 genera and more than 250 species, and organisms within this family include pathogenic bacteria capable of causing many different infectious processes, such as sepsis, pneumonia, urinary tract infections, intra-abdominal infections and foodborne illnesses. 16S gene sequencing, a gold standard for determining the species of a bacteria, however is not adequate due to strong conservation of sequences among members of this family across multiple genera. Instead, sequencing housekeeping genes such as dnaJ has been shown to distinguish species within this family.

We have designed an Enterobacterales PCR test (ENBDNA) based on partial dnaJ gene sequencing which can be used to detect and identify members of Enterobacterales order. This test is currently offered reflexively when 16S sequence (from BCTDNA or NGS16S) suggests presence of Enterobacteriaceae family DNA.

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms

16S sequencing, bacteria 16S, bacteria identification, Bacteria PCR, bacteria sequencing, bacterial 16S, bacterial identification, Bacterial PCR, bacterial sequencing, broad range bacteria PCR, broad range bacterial PCR, dnaJ, dnaJ identification, dnaJ PCR, dnaJ sequening, Enterobacteriaceae identification, Enterobacteriaceae sequencing, Family Enterobacteriaceae, molecular bacteria, molecular bacterial, Synonyms: 16S PCR, universal bacteria PCR, universal bacterial PCR

Components

Code	Name
ENBRS	Enterobacterales PCR: Detection, dnaJ
ENBID	Enterobacterales PCR: dnaJ Identification
ENBSI	Enterobacterales PCR: Specimen Description
ENBSPI	Enterobacterales PCR: External Identifier
ENBSR	Enterobacterales PCR: Special Requests
ENBSC	Enterobacterales PCR: Specimen Comments
ENBNAE	Enterobacterales PCR: Specimen DNA Extraction
ENBREV	Enterobacterales PCR: Pathologist Review
ENBME	Enterobacterales PCR: Method Note

Interpretation

Reference Range

See individual components

Guidelines

Specialty testing for Molecular Microbiology

Ordering & Collection

Specimen Type: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).
Collection: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).
Handling Instructions: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).
Quantity: requested: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).
minimum: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).

Processing

Processing: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).

Performance

LIS Dept Code

Micro Molecular Diag (MMD)

Performing Location(s)

UW-MT	Microbiology, Molecular Diagnostics 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Results in 2-3 business days after reflex from Bacterial PCR (BCTDNA) or Bacterial detection by 16S NGS (NGS16S).

Available STAT?

Billing & Coding

CPT codes: 87801
LOINC: 76575-0
Interfaced Order Code: UOW4340