Enterobacterales DNA Detection

General Information

Lab Name

Enterobacterales DNA Detection

Lab Code

ENBDNA

Epic Name

Enterobacterales DNA Detection

Description

Enterobacterales DNA Detection by PCR is only available as a reflexive test when results indicate the presence of bacteria in the Order Enterobacterales from Bacterial PCR reflex NGS [BCTDNA], Bacterial DNA Detection by PCR (without reflex to NGS) [NRBDNA] or Bacterial detection by 16S NGS [NGS16S].

Results in 2-3 business days after reflex.

The family Enterobacteriaceae (within the order Enterobacterales) contains over 60 genera and more than 250 species, and organisms within this family include pathogenic bacteria capable of causing many different infectious processes, such as sepsis, pneumonia, urinary tract infections, intra-abdominal infections and foodborne illnesses. 16S gene sequencing, a gold standard for determining the species of a bacteria, however is not adequate due to strong conservation of sequences among members of this family across multiple genera. Instead, sequencing housekeeping genes such as dnaJ has been shown to distinguish species within this family.

We have designed Enterobacterales DNA Detection [ENBDNA] based on partial dnaJ gene sequencing which can be used to detect and identify members of the Order Enterobacterales. This test is currently offered reflexively when 16S sequence (from Bacterial PCR reflex NGS [BCTDNA], Bacterial DNA Detection by PCR (without reflex to NGS) [NRBDNA] or Bacterial detection by 16S NGS [NGS16S]) suggests presence of order Enterobacterales DNA.

Synonyms

16S sequencing, bacteria 16S, bacteria identification, Bacteria PCR, bacteria sequencing, bacterial 16S, bacterial identification, Bacterial PCR, bacterial sequencing, broad range bacteria PCR, broad range bacterial PCR, dnaJ, dnaJ identification, dnaJ PCR, dnaJ sequening, Enterobacteriaceae identification, Enterobacteriaceae sequencing, Family Enterobacteriaceae, molecular bacteria, molecular bacterial, Synonyms: 16S PCR, universal bacteria PCR, universal bacterial PCR

Components

Code	Name
ENBRS	Enterobacterales PCR: Detection, dnaJ
ENBID	Enterobacterales PCR: dnaJ Identification
ENBSI	Enterobacterales PCR: Specimen Description
ENBSPI	Enterobacterales PCR: External Identifier
ENBSR	Enterobacterales PCR: Special Requests
ENBSC	Enterobacterales PCR: Specimen Comments
ENBNAE	Enterobacterales PCR: Specimen DNA Extraction
ENBREV	Enterobacterales PCR: Pathologist Review
ENBME	Enterobacterales PCR: Method Note