Mycobacterium chimaera DNA detection by PCR

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General Information

Lab Name
Mycobacterium chimaera DNA detection by PCR
Lab Code
MCHDNA
Epic Ordering
Mycobacterium chimaera DNA detection by PCR
Description

Mycobcaterium chimaera DNA detection by PCR is only available upon request as a reflexive test if a 3-way split ID of “Mycobacterium chimaera or Mycobacterium intracellularae or Mycobacterium paraintracellularae” is reported from tissue/fluid or a pure culture.

Results in 2-3 business days after reflex.

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms
Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB sequencing, M. avium complex, MAC, MAI PCR, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterium chimaera, Mycobacterium identification
Components

Interpretation

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Tissue (Fresh frozen or paraffin-embedded), Fluid (see Acceptable Specimens for details), Pure Culture
Collection

Acceptable Specimens

For tissue/fluid acceptable specimens please refer to corresponding battery that this test can be reflexed from. Each of the following batteries have a variation in acceptable specimens.

Pure Culture

  • Plate/slant: should have visible growth
  • Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
  • A mixed culture will be subject to an additional charge

Optimal Quantity

For tissue/fluid Optimal Quantity please refer to corresponding battery that this test can be reflexed from.

Handling Instructions

Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.

Shipping/Handling

  • Fresh frozen tissue/fluid specimens should be collected into a DNA free container labeled with at least two identifiers and be submitted and maintained on dry ice.
  • Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
  • Pure cultures* should be sent at room or refrigerated temperature. Follow approved regulations for transporting infectious substances.

*Plate/slant: should have visible growth
*Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis


Quantity
requested: See Optimal Quantity above
minimum: Specimens below optimal quantity are acceptable for testing, however, diagnostic yield is generally proportional to specimen size.

Processing

Processing

UWMC/HMC: Store and send fresh tissue/fluid specimens refrigerated, if specimen storage and transport will exceed 8 hours, freeze at -20°C. Freeze all fresh tissue/fluid specimens at -20°C upon arrival in UW Molecular Microbiology.

Performance

LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
UW-MT Microbiology, Molecular Diagnostics
206-520-4600

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Shipping Address

Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW Room 117
Renton, WA 98057
Phone: 206-520-4600
Alternate phone: 206-598-6147

Performing Lab Address

Clinical Microbiology Lab, NW177
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195
Phone: 206-598-5735
Alternate phone: 206-598-6147

Contact Information

Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible.

email: molmicdx@uw.edu

The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT.

Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198.

For results or other inquiries, we can be reached by phone at the following numbers:

  • Phone: (206) 598-5735
  • Alternate phone: (206) 598-6147
  • FAX: (206) 520-4903

For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190

Frequency
Results in 2-3 business days after reflex.
Available STAT?
No

Billing & Coding

CPT codes
87551
LOINC
14974-0
Interfaced Order Code
UOW4632