Mycobacterium chimaera DNA detection by PCR

Interpretation

Guidelines

• Molecular Microbiology Acceptable Specimens
• Molecular Microbiology Specimen Submission
• Specialty testing for Molecular Microbiology

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range

See individual components

Ordering & Collection

Specimen Type

Tissue (Fresh frozen or paraffin-embedded), Fluid (see Acceptable Specimens for details), Pure Culture

Collection

Acceptable Specimens

For tissue/fluid acceptable specimens please refer to corresponding battery that this test can be reflexed from. Each of the following batteries have a variation in acceptable specimens.

• Nontuberculous Mycobacteria DNA Detection [NTMDNA]
• Mycobacterium avium complex DNA Detection [MAVDNA]
• Bacterial PCR reflex NGS [BCTDNA]

Pure Culture

• Plate/slant: should have visible growth
• Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
• A mixed culture will be subject to an additional charge
  
  

Optimal Quantity

For tissue/fluid Optimal Quantity please refer to corresponding battery that this test can be reflexed from.

• Nontuberculous Mycobacteria DNA Detection [NTMDNA]
• Mycobacterium avium complex DNA Detection [MAVDNA]
• Bacterial PCR reflex NGS [BCTDNA]

Handling Instructions

Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.

Shipping/Handling

• Fresh frozen tissue/fluid specimens should be collected into a DNA free container labeled with at least two identifiers and be submitted and maintained on dry ice.
• Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
• Pure cultures* should be sent at room or refrigerated temperature. Follow approved regulations for transporting infectious substances.

*Plate/slant: should have visible growth
*Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis

Quantity

Requested: See Optimal Quantity above
Minimum: Specimens below optimal quantity are acceptable for testing, however, diagnostic yield is generally proportional to specimen size.

Processing

Receiving Instructions

UWMC/HMC: Store and send fresh tissue/fluid specimens refrigerated, if specimen storage and transport will exceed 8 hours, freeze at -20°C. Freeze all fresh tissue/fluid specimens at -20°C upon arrival in UW Molecular Microbiology.

Misc Sendout

Performance

Lab Department

Micro Molecular Diag(MMD)

Frequency

Results in 2-3 business days after reflex.

Available STAT?

No

Performing Location(s)

UW-MT	Microbiology, Molecular Diagnostics 206-520-4600 ---------------------------------------- Shipping Address Attn: Molecular Microbiology UW CLSPS 1601 Lind Ave SW Room 117 Renton, WA 98057 Phone: 206-520-4600 Alternate phone: 206-598-6147 Performing Lab Address Clinical Microbiology Lab, NW177 University of Washington Medical Center 1959 NE Pacific Street Seattle, WA 98195 Phone: 206-598-5735 Alternate phone: 206-598-6147	Contact Information Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible. email: molmicdx@uw.edu The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT. Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198. For results or other inquiries, we can be reached by phone at the following numbers: Phone: (206) 598-5735 Alternate phone: (206) 598-6147 FAX: (206) 520-4903 For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190

Billing & Coding

CPT Codes

87551

LOINC

14974-0

Interfaced Order Code

UOW4632