Mycobacterium chimaera DNA detection by PCR

General Information

Lab Name

Lab Code

MCHDNA

Epic Ordering

Mycobacterium chimaera DNA detection by PCR

Description

Mycobcaterium chimaera DNA detection by PCR is only available upon request as a reflexive test if a 3-way split ID of “Mycobacterium chimaera or Mycobacterium intracellularae or Mycobacterium paraintracellularae” is reported from tissue/fluid or a pure culture.

Results in 2-3 business days after reflex.

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms

Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB sequencing, M. avium complex, MAC, MAI PCR, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterium chimaera, Mycobacterium identification

Components

Code	Name
MCSRRS	M chimaera PCR: Detection
MCHSI	M chimaera PCR: Specimen Description
MCHSPI	M chimaera PCR: External Identifier
MCHSR	M chimaera PCR: Special Requests
MCHSC	M chimaera PCR: Specimen Comments
MCHNAE	M chimaera PCR: Specimen DNA Extraction
MCHREV	M chimaera PCR: Pathologist Review
MCHME	M chimaera PCR: Method Note

Interpretation

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range

See individual components

Guidelines

Ordering & Collection

Specimen Type

Tissue (Fresh frozen or paraffin-embedded), Fluid (see Acceptable Specimens for details), Pure Culture

Collection

Acceptable Specimens

For tissue/fluid acceptable specimens please refer to corresponding battery that this test can be reflexed from. Each of the following batteries have a variation in acceptable specimens.

Pure Culture

Plate/slant: should have visible growth
Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
A mixed culture will be subject to an additional charge

Optimal Quantity

For tissue/fluid Optimal Quantity please refer to corresponding battery that this test can be reflexed from.

Handling Instructions

Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.

Shipping/Handling

Fresh frozen tissue/fluid specimens should be collected into a DNA free container labeled with at least two identifiers and be submitted and maintained on dry ice.
Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
Pure cultures* should be sent at room or refrigerated temperature. Follow approved regulations for transporting infectious substances.

*Plate/slant: should have visible growth
*Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis

Quantity

requested: See Optimal Quantity above
minimum: Specimens below optimal quantity are acceptable for testing, however, diagnostic yield is generally proportional to specimen size.

Processing

Processing: UWMC/HMC: Store and send fresh tissue/fluid specimens refrigerated, if specimen storage and transport will exceed 8 hours, freeze at -20°C. Freeze all fresh tissue/fluid specimens at -20°C upon arrival in UW Molecular Microbiology.

Performance

LIS Dept Code

Micro Molecular Diag (MMD)

Performing Location(s)

UW-MT	Microbiology, Molecular Diagnostics 206-520-4600 ---------------------------------------- Shipping Address Attn: Molecular Microbiology UW CLSPS 1601 Lind Ave SW Room 117 Renton, WA 98057 Phone: 206-520-4600 Alternate phone: 206-598-6147 Performing Lab Address Clinical Microbiology Lab, NW177 University of Washington Medical Center 1959 NE Pacific Street Seattle, WA 98195 Phone: 206-598-5735 Alternate phone: 206-598-6147	Contact Information Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible. email: molmicdx@uw.edu The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT. Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198. For results or other inquiries, we can be reached by phone at the following numbers: Phone: (206) 598-5735 Alternate phone: (206) 598-6147 FAX: (206) 520-4903 For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190

Frequency

Results in 2-3 business days after reflex.

Available STAT?

Billing & Coding

CPT codes: 87551
LOINC: 14974-0
Interfaced Order Code: UOW4632