AFB ID by Sequence Analysis

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General Information

Lab Name
AFB ID by Sequence Analysis
Lab Code
ASEQID
Epic Ordering
AFB ID by Sequence Analysis
Description

Culture that is identified as Mycobacterium tuberculosis (MTB), MTB complex, or M. bovis will have susceptibilities reflexively ordered for an additional charge. Blood bottles and trek broth must have organisms visible by stain to be acceptable for sequence analysis.

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms
Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB PCR from pure culture, AFB sequencing, bacteria 16S, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterial identification of isolated colonies, Mycobacterium identification
Components

Interpretation

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Pure Culture
Collection
Handling Instructions

Send pure culture (plate, tube or positive blood culture bottle) at room or refrigerated temperature. Follow approved regulations for transporting infectious substances. A mixed culture will be subject to an additional charge.

Quantity
requested: Plate, slant or bottle

Processing

Processing

Performance

LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
UW-MT Microbiology, Molecular Diagnostics
206-520-4600

Clinical Lab, Room # NW177,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Frequency
Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.
Available STAT?
No

Billing & Coding

CPT codes
87153
LOINC
14974-0
Interfaced Order Code
UOW4474