Lymphocyte subsets, T and B cell counts

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General Information

Lab Name
Lymphocyte subsets, T and B cell counts
Lab Code
TCSP
Epic Ordering
T Cell Subsets w/B Cells
Description

This assay provides absolute cell counts and percentages for CD4, CD8 and CD3 T cell subsets and CD19 B cell subsets.

All lymphocyte subset panels offered:
T cell subsets CD4/CD8 only, counts [TCS48] - CD4, CD8
T cell subsets CD3/CD4/CD8, counts [TCSA] - CD4, CD8, CD3
Lymphocyte subsets, T and B cell counts [TCSP] - CD4, CD8, CD3, CD19
Lymphocyte subsets, T, B, NK cell counts [TCSNK] - CD4, CD8, CD3, CD19, CD56
CD19+ B Cell Count [BCS19] - CD19 only

References

https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.21360

Synonyms
T & B Lymphocytes
Components

Interpretation

Method

This is a single platform lymphocyte subset assay and has been approved by the U.S. Food and Drug Administration.

Reference Range
See individual components
Ref. Range Notes
%CD4 [33-61]
Abs CD4 [0.730-2.250] Thou/ul
%CD8 [14-35]
Abs CD8 [0.250-1.240] Thou/ul
CD4/CD8 [1.00-3.78]
%CD3 [57-87]
Abs CD3 [1.040-3.400] Thou/ul
%CD19 [9-23]
Abs CD19 [0.160-0.390] Thou/ul
Interferences and Limitations

The stability limit for this FDA approved assay is 24 hours

References

https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.21360

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 or 5 mL Lavender (EDTA) tube
Unacceptable: GEL SEPARATOR

Handling Instructions

Specimens must be tested within 24 hours of collection for this FDA assay. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Specimens must be transported at room temperature. Refrigerated or frozen specimens will be canceled and a re-draw will be requested.

Quantity
requested: 3-4 ml
minimum: 1 ml

Processing

Processing

Stability: 24 hours @Room Temperature

SPS: Sample can be shared with CBC. Do not add on test if sample will be received in the Hematopathology lab outside the 24-hour stability limit. If sample is approaching 24 hour stability limit, contact hematopathology lab (206-606-7060) to notify them that a specimen is coming and send immediately.

Outside Laboratories: Specimens must be tested within 24 hours of collection. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Contact Lab Med Call Center 206-685-6066 to discuss transportation options. Specimens can be sent directly to the hematopathology lab as well (include contact info).

Performance

LIS Dept Code
Hematopathology (HP)
Performing Location(s)
Other Hematopathology
206-606-7060

UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109

Hematopathology hours:
5:30 am - 6:30 pm Monday through Saturday
7:30 am - 4:00 pm Sunday, and with reduced staffing on major holidays
Frequency
Monday-Sunday.
Available STAT?
No

Billing & Coding

CPT codes
86355, 86359, 86360
LOINC
18768-2
Interfaced Order Code
UOW4016