Anti DFS70

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General Information

Lab Name
Anti DFS70
Lab Code
ADFS70
Description

Used as a follow-up to samples that are ANA by IFA positive (homogenous or homogenous/speckled), but are negative/indeterminate for all specific ANA related antibodies.

The presence of anti-nuclear antibodies (ANA) and antibodies to extractable nuclear antigens (ENA) is a hallmark of systemic autoimmune rheumatic diseases (SARD). Indirect immunofluorescence (IIFA) testing on HEP2 cells is recommended as the ANA screening test of choice by the American College of Rheumatology. Up to 20% of healthy adults have been reported to have a positive ANA test. The DFS IIFA pattern has been reported in 33% of ANA-positive healthy individuals, but not in ANA-positive SARD patients. IIFA patterns which indicate the presence of anti-DFS70 antibodies should be assayed for the detection of anti-DFS70 antibodies. However, a positive anti-DFS70 does not rule out the presence of SARD.

References

Conrad et al., The Clinical Relevance of Anti-DFS70 Autoantibodies, Clin Rev Allergy Immunol (2017) 52:202.

Seelig et al., Autoantibodies Against DFS70/LEDGF Exclusion Markers for Systemic Autoimmune Rheumatic Diseases (SARD), Clin Lab (2016) 62:499.

Synonyms
Dense fine speckled antibody, LEDGF
Components

Interpretation

Method

ELISA that measures IgG antibodies to the DFS70 antigen (also known as LEDGF).

Reference Range
See individual components
Ref. Range Notes

Based on testing of 200 healthy blood donor samples during method validation in 2014.

References

Conrad et al., The Clinical Relevance of Anti-DFS70 Autoantibodies, Clin Rev Allergy Immunol (2017) 52:202.

Seelig et al., Autoantibodies Against DFS70/LEDGF Exclusion Markers for Systemic Autoimmune Rheumatic Diseases (SARD), Clin Lab (2016) 62:499.

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in RED TOP or SST tube

Handling Instructions

Centrifuge and separate serum. Refrigerate serum.

Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C or colder: Long Term

Instructions for Outside Clients only: Freeze serum @ -20°C

Quantity
requested: 1 mL serum
minimum: 0.5 mL serum

Processing

Processing

Stability: Room Temperature: up to 8 hours;

Refrigerated: 14 days;

Frozen at -20°C or colder: Long Term

Instructions for Outside Clients only: Freeze serum @ -20°C

Performance

LIS Dept Code
Immunology (IMM)
Performing Location(s)
UW-MT Immunology
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Frequency
Once a week
Available STAT?
No

Billing & Coding

CPT codes
86038
LOINC
47383-5
Interfaced Order Code
UOW3782