Anti DFS70
General Information
- Lab Name
- Anti DFS70
- Lab Code
- ADFS70
- Description
Used as a follow-up to samples that are ANA by IFA positive (homogenous or homogenous/speckled), but are negative/indeterminate for all specific ANA related antibodies.
The presence of anti-nuclear antibodies (ANA) and antibodies to extractable nuclear antigens (ENA) is a hallmark of systemic autoimmune rheumatic diseases (SARD). Indirect immunofluorescence (IIFA) testing on HEP2 cells is recommended as the ANA screening test of choice by the American College of Rheumatology. Up to 20% of healthy adults have been reported to have a positive ANA test. The DFS IIFA pattern has been reported in 33% of ANA-positive healthy individuals, but not in ANA-positive SARD patients. IIFA patterns which indicate the presence of anti-DFS70 antibodies should be assayed for the detection of anti-DFS70 antibodies. However, a positive anti-DFS70 does not rule out the presence of SARD.
- References
Conrad et al., The Clinical Relevance of Anti-DFS70 Autoantibodies, Clin Rev Allergy Immunol (2017) 52:202.
Seelig et al., Autoantibodies Against DFS70/LEDGF Exclusion Markers for Systemic Autoimmune Rheumatic Diseases (SARD), Clin Lab (2016) 62:499.
- Synonyms
- Dense fine speckled antibody, LEDGF
- Components
-
Code Name DFS70L Anti DFS70 Level DFS70C Anti DFS70 Comment DFS70M Anti DFS70 Method
Interpretation
- Method
ELISA that measures IgG antibodies to the DFS70 antigen (also known as LEDGF).
- Reference Range
- See individual components
- Ref. Range Notes
Based on testing of 200 healthy blood donor samples during method validation in 2014.
- References
Conrad et al., The Clinical Relevance of Anti-DFS70 Autoantibodies, Clin Rev Allergy Immunol (2017) 52:202.
Seelig et al., Autoantibodies Against DFS70/LEDGF Exclusion Markers for Systemic Autoimmune Rheumatic Diseases (SARD), Clin Lab (2016) 62:499.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL blood in RED TOP or SST tube
- Handling Instructions
Centrifuge and separate serum. Refrigerate serum.
Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C or colder: Long Term
Instructions for Outside Clients only: Freeze serum @ -20°C
- Quantity
-
Requested: 1 mL serum
Minimum: 0.5 mL serum
Processing
- Receiving Instructions
Stability: Room Temperature: up to 8 hours;
Refrigerated: 14 days;
Frozen at -20°C or colder: Long Term
Instructions for Outside Clients only: Freeze serum @ -20°C
- Misc Sendout
Performance
- Lab Department
- Immunology(IMM)
- Frequency
- Once a week
- Available STAT?
- No
- Performing Location(s)
-
UW-MT Immunology
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Billing & Coding
- CPT Codes
- 86038
- LOINC
- 47383-5
- Interfaced Order Code
- UOW3782