Anti DFS70

General Information

Lab Name

Anti DFS70

Lab Code

ADFS70

Description

Used as a follow-up to samples that are ANA by IFA positive (homogenous or homogenous/speckled), but are negative/indeterminate for all specific ANA related antibodies.

The presence of anti-nuclear antibodies (ANA) and antibodies to extractable nuclear antigens (ENA) is a hallmark of systemic autoimmune rheumatic diseases (SARD). Indirect immunofluorescence (IIFA) testing on HEP2 cells is recommended as the ANA screening test of choice by the American College of Rheumatology. Up to 20% of healthy adults have been reported to have a positive ANA test. The DFS IIFA pattern has been reported in 33% of ANA-positive healthy individuals, but not in ANA-positive SARD patients. IIFA patterns which indicate the presence of anti-DFS70 antibodies should be assayed for the detection of anti-DFS70 antibodies. However, a positive anti-DFS70 does not rule out the presence of SARD.

References

Conrad et al., The Clinical Relevance of Anti-DFS70 Autoantibodies, Clin Rev Allergy Immunol (2017) 52:202.

Seelig et al., Autoantibodies Against DFS70/LEDGF Exclusion Markers for Systemic Autoimmune Rheumatic Diseases (SARD), Clin Lab (2016) 62:499.

Synonyms

Dense fine speckled antibody, LEDGF

Components

Code	Name
DFS70L	Anti DFS70 Level
DFS70C	Anti DFS70 Comment
DFS70M	Anti DFS70 Method

Interpretation

Method: ELISA that measures IgG antibodies to the DFS70 antigen (also known as LEDGF).
Reference Range: See individual components
Ref. Range Notes: Based on testing of 200 healthy blood donor samples during method validation in 2014.

Ordering & Collection

Specimen Type: Blood
Collection: 3 mL blood in RED TOP or SST tube
Handling Instructions: Centrifuge and separate serum. Refrigerate serum.

Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C or colder: Long Term

Instructions for Outside Clients only: Freeze serum @ -20°C
Quantity: requested: 1 mL serum
minimum: 0.5 mL serum

Processing

Processing: Stability: Room Temperature: up to 8 hours;

Refrigerated: 14 days;

Frozen at -20°C or colder: Long Term

Instructions for Outside Clients only: Freeze serum @ -20°C

Performance

LIS Dept Code

Immunology (IMM)

Performing Location(s)

UW-MT	Immunology 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Once a week

Available STAT?

Billing & Coding

CPT codes: 86038
LOINC: 47383-5
Interfaced Order Code: UOW3782