Trypanosoma cruzi Total Antibody (Sendout)
General Information
- Lab Name
- Trypanosoma cruzi Total Antibody (Sendout)
- Lab Code
- RCHAGS
- Epic Name
- Trypanosoma cruzi Total Antibody (Sendout)
- External Test Id
- CRUZI
- Description
Useful For:
- Diagnosis of chronic Trypanosoma cruzi infection (Chagas disease)
Ordering Guidance: This is the preferred initial serologic test for diagnosis of Trypanosoma cruzi infection. The CDC recommends performing 2 independent tests for the serologic diagnosis of Chagas disease¹. See test RTRYPG (Trypanosoma cruzi Antibody, IgG) to ARUP Laboratories for a second independent assay.
***Trypanosoma cruzi Antibody by ELISA is no longer available separately at Mayo Clinic Laboratories. It will be performed as part of a panel, Trypanosoma cruzi (Chagas) Antibody Panel, Serum, along with the T. cruzi lateral flow assay.***
- References
- Centers for Disease Control and Prevention (CDC): Laboratory Diagnosis of American Trypanosomiasis
- Synonyms
- American trypanosomiasis, Chagas, Chagas disease, CHAGS, CRUZI, Kissing bug, Reduviid, T. cruzi, Triatoma, Trypanosoma, Trypanosomiasis
- Components
-
Code Name RCHAGR Trypanosoma cruzi Total Antibody
Interpretation
- Guidelines
- Method
Enzyme-Linked Immunosorbent Assay (ELISA)
- Reference Range
- See individual components
- Ref. Range Notes
Reference Value: Negative.
Interpretation:
- Negative: No antibodies to Trypanosoma cruzi (Chagas disease) detected. Antibodies to T cruzi may be absent during acute infection, therefore a negative result cannot be used to rule out recent infection. Repeat testing in 3 to 4 weeks is recommended if clinically indicated.
- Indeterminate: Repeat testing in 2 to 3 weeks if clinically indicated.
- Reactive: Antibodies to T cruzi detected. Result is not diagnostic. Supplemental testing by a second, different T cruzi serologic assay is recommended.
- Interferences and Limitations
Cautions:
False-positive results may occur in patients infected with Leishmania or other Trypanosoma species, including Trypanosoma rangeli.
Diagnosis of chronic Chagas disease requires both clinical evaluation (including exposure history) and laboratory testing. Chagas disease should not be diagnosed based on a single serologic result alone.
A single negative result does not exclude the diagnosis of Chagas disease as antibodies to the pathogen may not yet be detectable. Sensitivity of the assay may be decreased in significantly immunosuppressed patients.
- References
- Centers for Disease Control and Prevention (CDC): Laboratory Diagnosis of American Trypanosomiasis
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL blood in GOLD SST or RED TOP tube
- Handling Instructions
Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C.
Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.
Reject Due To: Gross hemolysis, gross lipemia.
- Quantity
-
Requested: 0.7 mL serum
Minimum: 0.6 mL serum
Processing
- Receiving Instructions
Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C.
Sendouts:
- Temporary workflow:
- Place a manual order in MayoAccess for Mayo Test: CRUZI
Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.
Reject Due To: Gross hemolysis, gross lipemia.
- Temporary workflow:
- Misc Sendout
Performance
- Lab Department
- Sendouts Mayo Lab (FZ)(MAFZ)
- Frequency
- Performed: Monday. Report Available: 1-8 days.
- Available STAT?
- No
- Performing Location(s)
-
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-17103050 Superior Drive NW
Rochester, MN 55901
Billing & Coding
- CPT Codes
- 86753
- LOINC
- 57320-4