Trypanosoma cruzi Total Antibody (Sendout)

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General Information

Lab Name
Trypanosoma cruzi Total Antibody (Sendout)
Lab Code
RCHAGS
Epic Ordering
Trypanosoma cruzi Total Antibody (Sendout)
External Test Id
CHAGS
Description

Useful For:

  • Diagnosis of chronic Trypanosoma cruzi infection (Chagas disease)

Ordering Guidance: This is the preferred initial serologic test for diagnosis of Trypanosoma cruzi infection. The CDC recommends performing 2 independent tests for the serologic diagnosis of Chagas disease¹. See test RTRYPG (Trypanosoma cruzi Antibody, IgG) to ARUP Laboratories for a second independent assay.

References
  1. Centers for Disease Control and Prevention (CDC): Laboratory Diagnosis of American Trypanosomiasis
Synonyms
American trypanosomiasis, Chagas, Chagas disease, CHAGS, Kissing bug, Reduviid, T. cruzi, Triatoma, Trypanosoma, Trypanosomiasis
Components

Interpretation

Method

Enzyme-Linked Immunosorbent Assay (ELISA)

Reference Range
See individual components
Ref. Range Notes

Reference Value: Negative.

Interpretation:

  • Negative: No antibodies to Trypanosoma cruzi (Chagas disease) detected. Antibodies to T cruzi may be absent during acute infection, therefore a negative result cannot be used to rule out recent infection. Repeat testing in 3 to 4 weeks is recommended if clinically indicated.
  • Indeterminate: Repeat testing in 2 to 3 weeks if clinically indicated.
  • Reactive: Antibodies to T cruzi detected. Result is not diagnostic. Supplemental testing by a second, different T cruzi serologic assay is recommended.
Interferences and Limitations

Cautions:

False-positive results may occur in patients infected with Leishmania or other Trypanosoma species, including Trypanosoma rangeli.

Diagnosis of chronic Chagas disease requires both clinical evaluation (including exposure history) and laboratory testing. Chagas disease should not be diagnosed based on a single serologic result alone.

A single negative result does not exclude the diagnosis of Chagas disease as antibodies to the pathogen may not yet be detectable. Sensitivity of the assay may be decreased in significantly immunosuppressed patients.

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in GOLD SST or RED TOP tube

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C.

Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia.

Quantity
requested: 0.7 mL serum
minimum: 0.6 mL serum

Processing

Processing

Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C.

Sendouts:

  • Order Mayo Test: CHAGS.
  • Interfaced: Yes.

Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia.

Performance

LIS Dept Code
Sendouts Mayo Lab (FZ) (MAFZ)
Performing Location(s)
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901

Frequency
Performed: Monday. Report Available: 1-8 days.
Available STAT?
No

Billing & Coding

CPT codes
86753
LOINC
57320-4