Histamine, Plasma

General Information

Lab Name
Lab Code: 165
Description: ***This test requires approval by the Lab Medicine resident on-call (206) 598-6190.*** For evaluating patients for diseases of immediate hypersensitivity, the preferred screening test is serum tryptase (see Lab mnemonic RTRYPT). Test results highly dependent on proper sample collection. Histamine may be released ex vivo by passing basophils in blood through a small-bore needle under vacuum or when blood clots. Serum should not be used in order to avoid the artifactual release of histamine from basophils that can occur during clotting.
Synonyms: Anaphylaxis, Immediate hypersensitivity

Specimen Type: plasma
Collection: One 3 mL lavender (EDTA) or pink (K2EDTA). Specimens must be collected in a pre-chilled tube and on ice.
Approval Required: This test requires approval by the Laboratory Medicine Resident on-call. Contact via the paging operator at 206-598-6190.
Handling Instructions: Separate specimens must be submitted when multiple tests ordered.
Quantity: requested: 1 mL plasma
minimum: 0.5 mL plasma

Processing

Centrifuge at 2-8°C and separate upper two-thirds of plasma within 20 minutes. Transfer 1 mL plasma to a plastic tube. Freeze immediately at -20°C.

Login: SEND1-FREEZE

Sendouts, order ARUP Test: 0070036

Unacceptable Conditions: Nonfrozen or hemolyzed specimens.

Stability: After separation from cells: Frozen (Critical): 6 months; Refrigerated: 6 hours; Ambient: Unacceptable.

LIS Dept Code

Performing Location(s)

Sendout	ARUP Laboratories, Inc. 800-522-2787 500 Chipeta Way Salt Lake City, UT 84108

Frequency

Tuesday,Saturday; Reported within 1-6 days

Available STAT?