Cytomegalovirus Rapid Detection

General Information

Lab Name

Rapid CMV by Shell Vial

Lab Code

RPDCMV

Epic Name

Rapid CMV by Shell Vial

Description

NOTE: This test is temporarily unavailable as of 03/25/2020.

The detection of viable Cytomegalovirus (CMV) prior to visible cytopathic effect (CPE) in conventional tube tissue culture. Specimens are inoculated into two 48-well plates containing a monolayer of a receptive cell line. At day 1 and 2 post inoculation, one plate is stained with a monoclonal antibody to CMV, and then reacted with fluorescent-conjugated goat anti-mouse immunoglobulin G. The cells are examined for characteristic nuclear staining which indicates the presence of adenovirus growth. Results are reported as CYTOMEGALOVIRUS DETECTED or Not detected.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

This code is only to be used if a viral culture has been already ordered.

The correct order codes are:

Herpes Group Culture and CMV Rapid Detection [VCCMVR] for samples requiring herpes group virus culture only or

Viral Culture and Rapid CMV Detection [VCSCMV] for samples that also require respiratory virus culture.

Synonyms

CMV Rapid Detection, Cytomegalovirus Culture Rapid Detection

Components

Code	Name
SPDES4	Viral Culture Specimen
RDAY1	CMV Rapid Detection Day 1
RDAY2	CMV Rapid Detection Day 2

Interpretation

Method: Tissue culture.
Reference Range: See individual components

Ordering & Collection

Specimen Type: Body fluids (CSF, BAL, Washes, Urine, Sputum), Swabs, Tissue, and Stool
Collection: Body fluids (CSF, BAL, Washes, Urine, Sputum): 1 mL of sample in sterile container

NP/Conjunctiva swabs: use FLEXIBLE, MINITIP FLOCKED SWAB in 3 ml universal transport media (UTM).
UWMC - PMM 304528, HMC - 51264, Community - Copan 305C or DHI 403C

Other Swabbed sites: use REGULAR FLOCKED SWAB in 3 ml universal transport media UTM.

Tissue: in sterile saline or universal transport media (UTM).

Stool: Pea size sample collected in a sterile container.
Quantity: Requested: See Collection
Minimum: 1 mL

Processing

Receiving Instructions: Body fluids (CSF, BAL, Washes, Urine, Sputum): Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

NP/Conjunctiva swabs: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Other Swabbed sites: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Tissue: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Stool: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Outside Laboratories:

Ship samples on Dry Ice, do not freeze/thaw. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack), shipment time must not exceed 48 hours from collection. Samples stored at 2-8°C greater than 48 hours old may be rejected.

Unacceptable samples: Blood (serum/plasma), dry swabs.

Note: Freezing specimens at -20°C is NOT acceptable for viral culture.

Misc Sendout

Performance

Lab Department

Virology - OLD(VIRO)

Frequency

Monday-Friday: Specimen in lab by 5PM. Saturday, Sunday and Holidays: Specimen in Lab by 2PM

Available STAT?

Performing Location(s)

Other	Virology 206-685-8037 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Billing & Coding

LOINC: 43701-2
Interfaced Order Code: UOW559