Viral Culture and Rapid CMV Detection

General Information

Lab Name

Rapid CMV and Viral Culture

Lab Code

VCSCMV

Epic Name

Culture Viral Screen w/Rapid CMV

Description

NOTE: This test is temporarily unavailable as of 03/25/2020.

Viral Culture

Viral isolation in tissue culture provides a broad, unbiased approach to identify viral pathogens. Inoculated susceptible living cells (tissue culture) are monitored for presumptive identification by the appearance of cytopathic effect (CPE, characteristic changes in the cell monolayer due to propagating virus). Four variables affect the presumptive identification of viral isolates: number of days to produce CPE, cell line(s) affected, appearance of CPE and source of specimen. Presumptive identification is confirmed by virus specific polymerase chain reaction (PCR) assays or the presence of specific viral antigens by fluorescent antibody staining.

Viral pathogens identified by viral culture include Adenovirus, Parainfluenza viruses, Respiratory Syncytial Virus, Influenza Viruses, Enterovirus, Rhinovirus, Herpes Simplex virus, Varicella zoster virus, and Cytomegalovirus (CMV).

Rapid CMV

The detection of viable CMV prior to visible cytopathic effect (CPE) in conventional tube tissue culture. Specimens are inoculated into two 48-well plates containing a monolayer of a receptive cell line. At day 1 and 2 post inoculation, one plate is stained with a monoclonal antibody to CMV, and then reacted with fluorescent-conjugated goat anti-mouse immunoglobulin G. The cells are examined for characteristic nuclear staining which indicates the presence of adenovirus growth. Results are reported as CYTOMEGALOVIRUS DETECTED or Not detected.

These tests were developed and their performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. They have not been cleared or approved by the US Food and Drug Administration.

For Viral Culture Screen only, see Viral Culture Screen [VCS]

For Herpes Group Culture (HSV1&2, CMV and VZV), see Herpes Group Culture (HSV 1&2, CMV, VZ) [VCHG]

For Herpes Group Culture and Rapid CMV, see Herpes Group Culture and CMV Rapid Detection [VCCMVR]

Synonyms

CMV Rapid Detection & Viral Culture Screen

Components

Code	Name
SPDES4	Viral Culture Specimen
RDAY1	CMV Rapid Detection Day 1
RDAY2	CMV Rapid Detection Day 2
PRLIM7	Viral Culture Prelim
FNL7	Viral Culture Final