The concentration of 1,25(OH)2D is determined by immunoaffinity extraction followed by liquid chromatography-tandem mass spectrometry. The reportable range is 5-200 pg/mL for both 1,25(OH)2D2 and 1,25(OH)2D3, which are summed to give the total 1,25(OH)2D concentration, with imprecision between 8.7 and 13.0 %CV.
Code | Name |
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125VD2 | 1,25 Dihydroxy Vitamin D2 |
125VD3 | 1,25 Dihydroxy Vitamin D3 |
125VDT | 1,25 Dihydroxy Vitamin D Total |
125VDM | 1,25 Dihydroxy Vitamin D Method |
Serum immunoaffinity extraction. Isotope dilution liquid chromatography mass spectrometry
All Locations:
Preferred: 4 mL blood in GOLD SST tube
Also Acceptable: 4 mL blood in ORANGE RST or RED TOP tube
Pediatric: 2 Full GOLD MICROTAINER
Testing is performed once weekly on Wednesday morning. Samples must be received by the performing lab by 5:00 a.m in order to be included in the weekly run. It is recommended that samples be collected prior to Wednesday morning in order to ensure that samples are included in the weekly run.
Freeze serum at -20°C
UW-MT |
Chemistry, General
206-520-4600 Clinical Lab, Room NW220, |
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