1,25 Dihydroxy Vitamin D

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General Information

Lab Name
1,25 Dihydroxy Vitamin D
Lab Code
VTD125
Epic Ordering
1,25 Dihydroxy Vitamin D
Description

The concentration of 1,25(OH)2D is determined by immunoaffinity extraction followed by liquid chromatography-tandem mass spectrometry. The reportable range is 5-200 pg/mL for both 1,25(OH)2D2 and 1,25(OH)2D3, which are summed to give the total 1,25(OH)2D concentration, with imprecision between 8.7 and 13.0 %CV.

Synonyms
Dihydroxyvitamin D
Components

Interpretation

Method

Serum immunoaffinity extraction. Isotope dilution liquid chromatography mass spectrometry

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Serum
Collection

All Locations:

Preferred: 4 mL blood in GOLD SST tube

Also Acceptable: 4 mL blood in ORANGE RST or RED TOP tube

Pediatric: 2 Full GOLD MICROTAINER

Handling Instructions

Testing is performed once weekly on Monday morning. Samples must be received by the performing lab by 5:00 a.m in order to be included in the weekly run. It is recommended that samples be collected prior to Monday morning in order to ensure that samples are included in the weekly run.

Quantity
requested: 1 mL serum
minimum: 0.8 mL serum

Processing

Processing

Freeze serum at -20°C

Performance

LIS Dept Code
Chemistry, University (CHU)
Performing Location(s)
UW-MT Chemistry, General
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Frequency
Monday, results next day
Available STAT?
No

Billing & Coding

CPT codes
82652
LOINC
49590-3
Interfaced Order Code
UOW1876