1,25 Dihydroxy Vitamin D
General Information
- Lab Name
- 1,25 Dihydroxy Vitamin D
- Lab Code
- VTD125
- Epic Ordering
- 1,25 Dihydroxy Vitamin D
- Description
The concentration of 1,25(OH)2D is determined by immunoaffinity extraction followed by liquid chromatography-tandem mass spectrometry. The reportable range is 5-200 pg/mL for both 1,25(OH)2D2 and 1,25(OH)2D3, which are summed to give the total 1,25(OH)2D concentration, with imprecision between 8.7 and 13.0 %CV.
- Synonyms
- Dihydroxyvitamin D
- Components
-
Code Name 125VD2 1,25 Dihydroxy Vitamin D2 125VD3 1,25 Dihydroxy Vitamin D3 125VDT 1,25 Dihydroxy Vitamin D Total 125VDM 1,25 Dihydroxy Vitamin D Method
Interpretation
- Method
Serum immunoaffinity extraction. Isotope dilution liquid chromatography mass spectrometry
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Serum
- Collection
-
All Locations:
Preferred: 4 mL blood in GOLD SST tube
Also Acceptable: 4 mL blood in ORANGE RST or RED TOP tube
Pediatric: 2 Full GOLD MICROTAINER
- Handling Instructions
Testing is performed once weekly on Monday morning. Samples must be received by the performing lab by 5:00 a.m in order to be included in the weekly run. It is recommended that samples be collected prior to Monday morning in order to ensure that samples are included in the weekly run.
- Quantity
-
requested: 1 mL serum
minimum: 0.8 mL serum
Processing
- Processing
Freeze serum at -20°C
Performance
- LIS Dept Code
- Chemistry, University (CHU)
- Performing Location(s)
-
UW-MT Chemistry, General
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195 - Frequency
- Monday, results next day
- Available STAT?
- No
Billing & Coding
- CPT codes
- 82652
- LOINC
- 49590-3
- Interfaced Order Code
- UOW1876