MTB complex PCR

General Information

Lab Name

MTB complex PCR

Lab Code

TBCPCR

Epic Ordering

Mycobacteria tuberculosis Complex PCR

Description

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms

MTBC, Mycobacterium tuberculosis complex PCR, TB Probe

Components

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range

See individual components

Guidelines

Specimen Type: Tissue (Fresh or paraffin embedded), Fluid (non-blood) from normally sterile sites, Brocheoalveolar-lavages (BALs), Urine, Processed sputa, Gastrointestinal biopsies
Collection: Specimens should be collected into a DNA free container labeled with at least two identifiers. Also accepted: E-swabs; Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids collected in Vacutainer tubes without preservative; LAVENDER top (EDTA) tube; tubes containing SPS (Yellow top)Note: Please indicate if sputa have been processed prior to submission for testing.Unacceptable: Tissues free-floating in formalin; samples containing SPS, citrate, or heparin; Blood/Serum specimens; samples collected on swabs (other than E-swabs)
Handling Instructions: Tissue specimens should be submitted and maintained on dry ice.
Quantity: requested: 1 mL Fluid; 1 cc Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.

Processing: UWMC/HMC – store and send specimen refrigerated and freeze specimens at -20°C upon arrival in UW-ML Microbiology. If specimen storage and transport will exceed 8 hours, freeze at -20°C.

Freeze specimens at -20°C upon arrival

Outside Laboratories: Freeze specimens in sterile containers at -20°C. Transport all specimens on ice.
Transport Temperature

LIS Dept Code

Microbiology (MC)

Performing Location(s)

UW-MT	Microbiology, Molecular Diagnostics 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.

Available STAT?