MTB complex PCR
General Information
- Lab Name
- MTB complex PCR
- Lab Code
- TBCPCR
- Epic Ordering
- Mycobacteria tuberculosis Complex PCR
- Description
For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website
- Synonyms
- MTBC, Mycobacterium tuberculosis complex PCR, TB Probe
- Components
-
Code Name SDES Specimen Description SREQ Special Requests CULT Culture RPT Report Status
Interpretation
- Method
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Tissue (Fresh or paraffin embedded), Fluid (non-blood) from normally sterile sites, Brocheoalveolar-lavages (BALs), Urine, Processed sputa, Gastrointestinal biopsies
- Collection
-
Specimens should be collected into a DNA free container labeled with at least two identifiers. Also accepted: E-swabs; Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids collected in Vacutainer tubes without preservative; LAVENDER top (EDTA) tube; tubes containing SPS (Yellow top)Note: Please indicate if sputa have been processed prior to submission for testing.Unacceptable: Tissues free-floating in formalin; samples containing SPS, citrate, or heparin; Blood/Serum specimens; samples collected on swabs (other than E-swabs)
- Handling Instructions
Tissue specimens should be submitted and maintained on dry ice.
- Quantity
-
requested: 1 mL Fluid; 1 cc Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.
Processing
- Processing
UWMC/HMC – store and send specimen refrigerated and freeze specimens at -20°C upon arrival in UW-ML Microbiology. If specimen storage and transport will exceed 8 hours, freeze at -20°C.
Freeze specimens at -20°C upon arrival
Outside Laboratories: Freeze specimens in sterile containers at -20°C. Transport all specimens on ice.
- Transport Temperature
Performance
- LIS Dept Code
- Microbiology (MC)
- Performing Location(s)
-
UW-MT Microbiology, Molecular Diagnostics
206-520-4600Clinical Lab, Room # NW177,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195 - Frequency
- Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.
- Available STAT?
- No
Billing & Coding
- CPT codes
- LOINC
- 38379-4