MTB complex PCR

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General Information

Lab Name
MTB complex PCR
Lab Code
TBCPCR
Epic Ordering
Mycobacteria tuberculosis Complex PCR
Description

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms
MTBC, Mycobacterium tuberculosis complex PCR, TB Probe
Components

Interpretation

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Tissue (Fresh or paraffin embedded), Fluid (non-blood) from normally sterile sites, Brocheoalveolar-lavages (BALs), Urine, Processed sputa, Gastrointestinal biopsies
Collection

Specimens should be collected into a DNA free container labeled with at least two identifiers. Also accepted: E-swabs; Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids collected in Vacutainer tubes without preservative; LAVENDER top (EDTA) tube; tubes containing SPS (Yellow top)Note: Please indicate if sputa have been processed prior to submission for testing.Unacceptable: Tissues free-floating in formalin; samples containing SPS, citrate, or heparin; Blood/Serum specimens; samples collected on swabs (other than E-swabs)

Handling Instructions

Tissue specimens should be submitted and maintained on dry ice.

Quantity
requested: 1 mL Fluid; 1 cc Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.

Processing

Processing

UWMC/HMC – store and send specimen refrigerated and freeze specimens at -20°C upon arrival in UW-ML Microbiology. If specimen storage and transport will exceed 8 hours, freeze at -20°C.

Freeze specimens at -20°C upon arrival

Outside Laboratories: Freeze specimens in sterile containers at -20°C. Transport all specimens on ice.

Transport Temperature

Performance

LIS Dept Code
Microbiology (MC)
Performing Location(s)
UW-MT Microbiology, Molecular Diagnostics
206-520-4600

Clinical Lab, Room # NW177,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Frequency
Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.
Available STAT?
No

Billing & Coding

CPT codes
LOINC
38379-4