Syphilis Serologic Confirmation (Sendout)

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General Information

Lab Name
Syphilis Serologic Confirmation (Sendout)
Lab Code
RSYPHC
Epic Name
Syphilis Serologic Confirmation (Sendout)
Description

Ordering Note: For use by UW-MT Immunology Laboratory only. Reflex supplementary testing for borderline/reactive Serologic Syphilis Screen [SYPHSC] results.

Testing Algorithm: CMIA (detection of IgG and IgM antibodies by Abbott chemiluminescent Immunoassay) and RPR (Rapid Plasma Reagin, qualitative and quantitative) are performed on each specimen. If the results are inconclusive or discrepant between the two tests, a confirmatory TP-PA (Treponema pallidum Particle Agglutination) is performed.

Forms & Requisitions
Components
Code Name
RTCMIA Syphilis Conf Treponemal CMIA, IgG/IgM
RRTPPA Syphilis Conf TPPA
RPRQNT Syphilis Conf RPR Quantitative Titer
RSYPPL Syphilis Conf Performing Lab
SYPHIF Final Syphilis Screening

Interpretation

Guidelines
Method

Treponemal Specific Assays:

  • CMIA (chemiluminescent Immunoassay): Detection of IgG and IgM antibodies by Abbott.
  • TP-PA: (Treponema pallidum Particle Agglutination) Confirmation of IgG and IgM antibodies by Fujirebio.

Non-Treponemal Specific Assay:

  • RPR: (Rapid Plasma Reagin) Qualitative and quantitative detection of non-specific antibodies by BD.
Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood
Collection

Orderable by reflex only.

Forms & Requisitions
Quantity
Requested: 2 mL serum
Minimum: 0.5 mL serum

Processing

Receiving Instructions

Immunology: If RSYPHC reflexes from SYPHSC, aliquot extra serum into a separate vial (labeled with RSYPHC Sunquest CID). Freeze serum at -20°C.

Sendouts:

  • Immunology will deliver serum aliquot with a completed WA PHL STI Requisition for sendout to the state lab.
  • Package each patient's sample in an individual biohazard bag with a copy of the associated requisition (one patient per bag).
    • Orders received with multiple patient samples/requisitions in the same biohazard bag may be rejected by WA PHL.
  • Send samples on dry ice with the DLMP courier.

WAPHL Specimen Collection and Submission Instructions: Syphilis, Reference

Stability: Frozen: Indefinitely; Refrigerated: 5 days; Ambient: Unacceptable.

Rejection Criteria: Unacceptable specimen type, not refrigerated or frozen, insufficient specimen volume, leaky specimen.

Misc Sendout

Performance

Lab Department
Serology Sendouts(SRSO)
Frequency
Monday - Friday, excluding holidays. TAT: CMIA and RPR: 3 business days. TP-PA: 5 business days.
Available STAT?
No
Performing Location(s)
Sendout Washington State Public Health Laboratory
206-418-5400

1610 NE 150th St.
Shoreline, WA 98155
Other Locations/Notes
  • WA PHL STI Lab: (206) 418-5622

Billing & Coding

CPT Codes
00000
LOINC
68502-4
Interfaced Order Code
UOW4592