Syphilis Serologic Confirmation (Sendout)

General Information

Lab Name

Lab Code

RSYPHC

Epic Name

Syphilis Serologic Confirmation (Sendout)

Description

Ordering Note: For use by UW-MT Immunology Laboratory only. Reflex supplementary testing for borderline/reactive Serologic Syphilis Screen [SYPHSC] results.

Testing Algorithm: CMIA (detection of IgG and IgM antibodies by Abbott chemiluminescent Immunoassay) and RPR (Rapid Plasma Reagin, qualitative and quantitative) are performed on each specimen. If the results are inconclusive or discrepant between the two tests, a confirmatory TP-PA (Treponema pallidum Particle Agglutination) is performed.

Forms & Requisitions

WAPHL STI Requisition Form

Components

Code	Name
RTCMIA	Syphilis Conf Treponemal CMIA, IgG/IgM
RRTPPA	Syphilis Conf TPPA
RPRQNT	Syphilis Conf RPR Quantitative Titer
RSYPPL	Syphilis Conf Performing Lab
SYPHIF	Final Syphilis Screening

Interpretation

Guidelines

Washington DOH Notifiable Conditions

Method

Treponemal Specific Assays:

CMIA (chemiluminescent Immunoassay): Detection of IgG and IgM antibodies by Abbott.
TP-PA: (Treponema pallidum Particle Agglutination) Confirmation of IgG and IgM antibodies by Fujirebio.

Non-Treponemal Specific Assay:

RPR: (Rapid Plasma Reagin) Qualitative and quantitative detection of non-specific antibodies by BD.

Reference Range

See individual components

Ordering & Collection

Specimen Type

Blood

Collection

Orderable by reflex only.

Forms & Requisitions

WAPHL STI Requisition Form

Quantity

Requested: 2 mL serum
Minimum: 0.5 mL serum

Processing

Receiving Instructions

Immunology: If RSYPHC reflexes from SYPHSC, aliquot extra serum into a separate vial (labeled with RSYPHC Sunquest CID). Freeze serum at -20°C.

Sendouts:

Immunology will deliver serum aliquot with a completed WA PHL STI Requisition for sendout to the state lab.
Package each patient's sample in an individual biohazard bag with a copy of the associated requisition (one patient per bag).
- Orders received with multiple patient samples/requisitions in the same biohazard bag may be rejected by WA PHL.
Send samples on dry ice with the DLMP courier.

WAPHL Specimen Collection and Submission Instructions: Syphilis, Reference

Stability: Frozen: Indefinitely; Refrigerated: 5 days; Ambient: Unacceptable.

Rejection Criteria: Unacceptable specimen type, not refrigerated or frozen, insufficient specimen volume, leaky specimen.

Misc Sendout

Performance

Lab Department

Serology Sendouts(SRSO)

Frequency

Monday - Friday, excluding holidays. TAT: CMIA and RPR: 3 business days. TP-PA: 5 business days.

Available STAT?

Performing Location(s)

Sendout	Washington State Public Health Laboratory 206-418-5400 1610 NE 150th St. Shoreline, WA 98155

Other Locations/Notes

WA PHL STI Lab: (206) 418-5622

Billing & Coding

CPT Codes: 00000
LOINC: 68502-4
Interfaced Order Code: UOW4592