Lymphocyte Mitogen/Ag Stimulation (Sendout)

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General Information

Lab Name
Lymphocyte Mitogen/Ag Stimulation
Lab Code
RMIAGP
Epic Ordering
Lymphocyte Mitogen/Ag Stimulation (Sendout)
External Test Id
LAB3317, LAB2806
Description

Description:

  • The Mitogen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to non-specific stimuli (PHA and/or anti-CD3).
  • The Antigen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to specific stimuli (Tetanus and/or Candida).

Ordering Requirements: Provider must specify which mitogens (PHA, CD3) and/or antigens (Tetanus, Candida) are required. Failure to do so may result in significant delays or cancellations.

Collection Note: Limited specimen stability. Time blood collections accordingly to ensure that samples reach the performing lab within the 24-hour stability limit.

Forms & Requisitions
Synonyms
anti-CD3, Antigen stimulation, Candida Antigen Stimulation, LAB3317, Lymphocyte Function Analysis, Lymphocyte Transformation, MSS, PHA, Phytohemagglutinin, T Cell proliferation to Antigens, T Cell proliferation to Mitogens, Tetanus Stimulation
Components

Interpretation

Method

Lymphocyte proliferation with 3H thymidine incorporation

Reference Range
See individual components
Ref. Range Notes

Reference Values: See report.

Ordering & Collection

Specimen Type
Blood
Collection

20 mL blood in (2) 10 mL GREEN TOP (Sodium Heparin/Na Hep) tubes

  • Expedite transport to the lab. Samples must arrive at SCHL within 24 hours of collection.
  • AM Collections: Collect Monday - Friday.
    • Samples collected on a Friday must arrive in SPS by 09:00 A.M. so that samples arrive at SCHL with sufficient time to make the run at noon.
  • PM collections: Collect Monday - Thursday only. Orders collected late morning or afternoon on Fridays will be cancelled.
  • Do not collect on or before holidays.

Unacceptable: Lithium heparin tubes (with or without gel)

Forms & Requisitions
Handling Instructions

Outside Laboratories: Due to the limited sample stability for this test, outside laboratories should arrange for testing directly with Seattle Children's Hospital Laboratory.

Quantity
requested: 20 mL whole blood (NaHep)
minimum: 20 mL whole blood (NaHep)

Processing

Processing

SPS: Expedite processing. Notify Sendouts staff upon receipt of samples in the lab. Store in ambient Sendouts rack (USENDA).

Login: at result prompt for RMIAGR, enter Mitogen(s) and/or Antigen(s) requested by provider. (Choices: PHA, CD3, Candida, Tetanus).

Sendouts:

  • Review EPIC order to confirm which mitogens and/or antigens are being requested.
  • Expedite processing. Send samples as received via Delivery Express courier.
    • AM Collections: Must be sent out same day and arrive in SCH Lab by noon for same-day setup.
    • PM Collections: May be held at room temperature for sendout the following morning. Must arrive at SCH Lab by noon for setup.
  • If samples are sent close to the noon cutoff for setup, notify SCHL Main Lab of incoming samples by calling 206-987-2617. Failure to do so may result in test cancellations.

Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.

Performance

LIS Dept Code
Performing Location(s)
Sendout Seattle Children's Hospital Department of Laboratories
206-987-2617

4800 Sand Point Way NE
OC.8.720
Seattle, WA 98105
Other Locations/Notes

Seattle Children's Hospital Cell Marker Laboratory:

  • Phone: 206-987-2560
Frequency
Performed: Monday-Thursday; Friday if specimen arrives at SCHL by noon. Results available in 7-10 days.
Available STAT?
No

Billing & Coding

CPT codes
86353
LOINC
59063-8
Interfaced Order Code
UOW3979