Lymphocyte Mitogen/Antigen Stimulation (Sendout)

Description:

• The Mitogen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to non-specific stimuli (PHA and/or anti-CD3).
• The Antigen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to specific stimuli (Tetanus and/or Candida).

Ordering Requirements: Provider must specify which mitogens (PHA, CD3) and/or antigens (Tetanus, Candida) are required. Failure to do so may result in significant delays or cancellations.

Collection Note: Limited specimen stability. Time blood collections accordingly to ensure that samples reach the performing lab within the 24-hour stability limit.

Collection: 20 mL blood in (2) 10 mL GREEN TOP (Sodium Heparin/Na Hep) tubes

• Unacceptable: Lithium heparin tubes (with or without gel)

Collection Timing:

• AM Collections: Collect Monday - Friday. Samples collected on a Friday must arrive in SPS by 09:00 A.M. to ensure sufficient time to make the noon run at Seattle Children's.
• PM collections: Collect Monday - Thursday only. Orders collected late morning or afternoon on Fridays will be cancelled.
• Do not collect on or before holidays.

Expedite transport to the lab. Samples must arrive at SCH Lab within 24 hours of collection.

Vancouver Clinic (TVC) Only:

• Expedite transport to the UW Central Lab to ensure that the samples arrive at Seattle Children's lab within the 24-hour stability.
• Include the mitogen(s) and/or antigen(s) requested for each order (PHA, CD3, Tetanus, Candida). Failure to provide this information may result in delays or cancellations.

Other Outside Laboratories: Due to the limited sample stability for this test, clients should arrange for testing directly with Seattle Children's Hospital Laboratory.

Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.

SPS: Short stability; expedite processing. Notify Sendouts staff upon receipt of samples in the lab. Store samples in the ambient Sendouts rack at each site (USENDA/HSENDA/CSENDA).

Result-at-Entry:

• RMIAGR (Lymphocyte Mitogen/Antigen Requested): <If not filed from Epic, enter the specific mitogens and/or antigens requested from the table below.>

Mitogens/Antigens Available:

Sendouts (UW-MT or HMC)/Central Lab (RCLSPS):

• SEACHL Tests:
  • LAB3317 (Mitogen Stimulation Study)
  • LAB2806 (Antigen Stimulation Study)
• Review the Epic order or TVC manifest to confirm which mitogens and/or antigens are being requested. Complete the SEACHL Cell Marker Requisition accordingly.
• Expedite transport. Send samples as received via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
  • AM Collections: Must be sent out same day and arrive in SCH Lab by noon for same-day setup.
  • PM Collections: May be held at room temperature for sendout the following morning. Must arrive at SCH Lab by noon for setup.
• Samples drawn on Friday must be received at SCH lab by noon.
• Notify SCH Cell Marker Lab at 206-987-2560 if sending a sample nearing the stability limit or Friday noon cutoff.

Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.

Seattle Children's Hospital Cell Marker Laboratory:

• Phone: 206-987-2560