Lyme Disease Antibody, Immunoblot (Sendout)

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General Information

Lab Name
Lyme Disease Antibody, Immunoblot (Sendout)
Lab Code
RLYMIB
Epic Ordering
Lyme Disease Antibody, Immunoblot (Sendout)
External Test Id
LYWB
Description

Useful For:

  • Aiding in the diagnosis of systemic Lyme disease.

  • This test should not be used as a screening assay.

Ordering Note: Reflex test to be added by Mayo for positive Lyme Disease serology tests (refer to separate entry, RLYMES). May also be ordered directly per provider request.

Synonyms
Borrelia burgdorferi, Lyme Disease Antibody, Lyme Disease Antibody Western Blot, Lyme Disease ImmunoBlot Assay, Lyme ImmunoBlot, Lyme Western Blot, Western Blot Assay
Components

Interpretation

Method

Immunoblot Microarray

Reference Range
See individual components
Ref. Range Notes

Reference Values:

IgG: Negative
IgM:

Negative

Interpretation:

IgM:

IgM antibodies to Borrelia burgdorferi may be detectable within 1 to 2 weeks following the tick bite; they usually peak during the third to sixth week after disease onset, and then demonstrate a gradual decline over a period of months. IgM antibody may persist for months following completion of treatment. IgM antibody results against B burgdorferi should only be considered during the 30 days following exposure and symptom onset.

Negative specimens typically demonstrate antibodies to fewer than 2 of the 3 significant B burgdorferi proteins. Additional specimens should be submitted in 2 to 3 weeks if B burgdorferi exposure has not been ruled out.

IgG:

IgG antibodies to B burgdorferi can be detected approximately 2 weeks after onset of disease and can remain detectable for months to years following completion of therapy.

Normal specimens and false-positive enzyme immunoassay (EIA) specimens generally have antibodies to 4 or fewer proteins. Except for early patients, antibodies from patients with Lyme disease generally bind to 5 or more proteins.

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in GOLD SST or RED TOP tube

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 14 days; Frozen: 30 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivated specimen.

Quantity
requested: 0.75 mL serum
minimum: 0.5 mL serum

Processing

Processing

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Sendouts:

  • Order Mayo Test: LYWB.
  • If ordered concurrently with RLYMES, test must be ordered manually in MayoAccess. Mayo will not accept an order for LYME and LYWB on the same accession.

Stability: Refrigerated (preferred): 14 days; Frozen: 30 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivated specimen.

Performance

LIS Dept Code
Sendouts Mayo Lab (RF) (MARF)
Performing Location(s)
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901

Frequency
Performed: Monday - Friday. Report Available: 1-4 days.
Available STAT?
No

Billing & Coding

CPT codes
86617x2
LOINC
18203-0
Interfaced Order Code
UOW4240