Lyme Disease Antibody, Immunoblot (Sendout)
General Information
- Lab Name
- Lyme Disease Antibody, Immunoblot (Sendout)
- Lab Code
- RLYMIB
- Epic Ordering
- Lyme Disease Antibody, Immunoblot (Sendout)
- External Test Id
- LYWB
- Description
Useful For:
-
Aiding in the diagnosis of systemic Lyme disease.
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This test should not be used as a screening assay.
Ordering Note: Reflex test to be added by Mayo for positive Lyme Disease serology tests (refer to separate entry, RLYMES). May also be ordered directly per provider request.
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- Synonyms
- Borrelia burgdorferi, Lyme Disease Antibody, Lyme Disease Antibody Western Blot, Lyme Disease ImmunoBlot Assay, Lyme ImmunoBlot, Lyme Western Blot, Western Blot Assay
- Components
-
Code Name RLDGIB IgG Immunoblot RLDGDA IgG detected against: RLDMIB IgM Immunoblot RLDMDA IgM detected against: RLDIBI Lyme Disease Immunoblot Interp
Interpretation
- Method
Immunoblot Microarray
- Reference Range
- See individual components
- Ref. Range Notes
Reference Values:
IgG: Negative IgM: Negative
Interpretation:
IgM:
IgM antibodies to Borrelia burgdorferi may be detectable within 1 to 2 weeks following the tick bite; they usually peak during the third to sixth week after disease onset, and then demonstrate a gradual decline over a period of months. IgM antibody may persist for months following completion of treatment. IgM antibody results against B burgdorferi should only be considered during the 30 days following exposure and symptom onset.
Negative specimens typically demonstrate antibodies to fewer than 2 of the 3 significant B burgdorferi proteins. Additional specimens should be submitted in 2 to 3 weeks if B burgdorferi exposure has not been ruled out.
IgG:
IgG antibodies to B burgdorferi can be detected approximately 2 weeks after onset of disease and can remain detectable for months to years following completion of therapy.
Normal specimens and false-positive enzyme immunoassay (EIA) specimens generally have antibodies to 4 or fewer proteins. Except for early patients, antibodies from patients with Lyme disease generally bind to 5 or more proteins.
- Guidelines
Ordering & Collection
- Specimen Type
- Blood
- Collection
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3 mL blood in GOLD SST or RED TOP tube
- Handling Instructions
Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Stability: Refrigerated (preferred): 14 days; Frozen: 30 days; Ambient: Unacceptable.
Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivated specimen.
- Quantity
-
requested: 0.75 mL serum
minimum: 0.5 mL serum
Processing
- Processing
Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Sendouts:
- Order Mayo Test: LYWB.
- If ordered concurrently with RLYMES, test must be ordered manually in MayoAccess. Mayo will not accept an order for LYME and LYWB on the same accession.
Stability: Refrigerated (preferred): 14 days; Frozen: 30 days; Ambient: Unacceptable.
Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivated specimen.
Performance
- LIS Dept Code
- Sendouts Mayo Lab (RF) (MARF)
- Performing Location(s)
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Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-17103050 Superior Drive NW
Rochester, MN 55901 - Frequency
- Performed: Monday - Friday. Report Available: 1-4 days.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 86617x2
- LOINC
- 18203-0
- Interfaced Order Code
- UOW4240