HLA Antibody Monitoring DSA, STAT (Sendout)

Indications: Solid organ transplant.

Test Information: The purpose of this test is to monitor patients after transplant for the presence and level of antibodies to the donor’s HLA antigens. The test is semi-quantitative.

Ordering Guidance: This test is appropriate when results will be used to evaluate treatment options, or when results are needed overnight or over the weekend. STAT testing includes a 6-hour turnaround time with verbal result reporting. If this is not required, select alternate test options HLA Antibody Monitoring DSA, Routine (Sendout) [RHLAM] or HLA Antibody Monitoring DSA, Protocol (Sendout) [RHLAMP], as appropriate.

Ordering Requirements:

• Providers must notify Bloodworks Northwest HLA Laboratory of any STAT requests by calling (206) 817-7730. If this is not done, the lab will perform the test as routine.
• Contact information is required for verbal result reporting. Include the name and phone/pager of the person receiving results in the Epic order.

10 mL blood in RED TOP (clot) tube

Unacceptable: GOLD SST

Outside Laboratories: To prevent testing delays, it is recommended that STAT orders are sent directly to Bloodworks Northwest. If sending through UW-MT:

• Transport whole blood samples at room temperature (serum from a red top tube is also accepted). Samples must be received by Bloodworks Northwest within 10 days of collection.
• A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

Store and transport whole blood samples at ambient temperature.

Sendouts:

• Bloodworks NW HLA Test: 3083-18
• Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
• Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
  
  • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
• STAT orders should be transported to Bloodworks Northwest as received. Depending on the DLMP courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.

Stability: Ambient: 10 days. Serum is also accepted.