HLA Antibody Monitoring DSA, Protocol (Sendout)

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General Information

Lab Name
HLA Antibody Monitoring DSA, Protocol (Sendout)
Lab Code
RHLAMP
Epic Name
HLA Antibody Monitoring DSA, Protocol (Sendout)
External Test Id
3083-23
Description

Indications: Solid organ transplant.

      Test Information: The purpose of this test is to monitor patients after transplant for the presence and level of antibodies to the donor’s HLA antigens. The test is semi-quantitative.

        Ordering Guidance (Protocol): This code is appropriate for DSA monitoring performed as part of a set protocol. Results will be available 6 business days after sample receipt. If a shorter turnaround time is required, order HLA Antibody Monitoring DSA, Routine (Sendout) [RHLAM] or HLA Antibody Monitoring DSA, STAT (Sendout) [RHLASM], as appropriate.

        References
        Forms & Requisitions
        Synonyms
        3083-23, Donor Specific Antibody, DSA, DSA Protocol, HLA Antibody Specificity-Monitoring DSA, Post-Transplant DSA
        Components
        Code Name
        RHLAMR HLA Antibody Monitoring DSA Interpretation

        Interpretation

        Method

        Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens

        Reference Range
        See individual components
        References

        Ordering & Collection

        Specimen Type
        Blood
        Collection

        10 mL blood in RED TOP (clot) tube

        Unacceptable: GOLD SST

        Forms & Requisitions
        Handling Instructions

        Outside Laboratories:

        • Transport whole blood samples at room temperature (serum from a red top tube is also accepted). Samples must be received by Bloodworks Northwest within 10 days of collection.
        • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

        Stability: Ambient: 10 days.

        Quantity
        Requested: 7-10 mL blood in red top tube
        Minimum: 2 mL blood in red top tube

        Processing

        Receiving Instructions

        Store and transport samples at ambient temperature.

        Sendouts (UW-MT/HMC) and FHCC SPS:

        • Bloodworks NW HLA Test: 3083-23
        • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
        • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
          • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
        • Send routine samples via scheduled DLMP Courier shipments (or Delivery Express, as needed).

        Stability: Ambient: 10 days. Serum is also accepted.

        Misc Sendout

        Performance

        Lab Department
        Sendouts BWNW Lab (AB)(BWAB)
        Frequency
        Performed: Monday through Friday. Reported: 2 business days from sample receipt at Bloodworks Northwest HLA Lab.
        Available STAT?
        No. Select test HLA Antibody Monitoring DSA, STAT (Sendout) [RHLASM] if STAT testing is required.
        Performing Location(s)
        Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
        206-689-6580

        921 Terry Ave
        6th Floor
        Seattle, WA 98104

        Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
        After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
        Fax: 206-689-6582

        Billing & Coding

        CPT Codes
        86834, 86835
        LOINC
        41618-0