Hepatitis D Antibodies, Total (Sendout)

Interpretation

Guidelines

• Washington DOH Notifiable Conditions

Method

Enzyme Immunoassay (EIA)

Reference Range

See individual components

Ref. Range Notes

Reference Values: Negative

Interpretation:

This assay detects the presence of hepatitis D virus (HDV)-specific total (combined IgG and IgM) antibodies in serum.

Negative results indicate the absence of HDV infection and no past exposure to HDV.

Equivocal results indicate borderline level of anti-HDV total antibodies. Repeat testing in 1 to 2 weeks is recommended to determine the definitive HDV infection status.

Positive results usually indicate one of the following conditions: 1) simultaneous acute or chronic coinfection with hepatitis B virus (HBV) and HDV, 2) acute HDV infection in patients with known chronic HBV infection (ie, HDV superinfection), or 3) resolved HDV infection. Results should be correlated with medical history and clinical findings.

Interferences and Limitations

Cautions:

Negative results may not rule-out hepatitis D virus (HDV) infection during the early phase of infection or in immunocompromised patients who have delayed or inadequate immune response.

False-positive results may be due to cross-reactive antibodies from other viral infection or underlying illnesses. Positive result should be correlated with the patient's clinical history, physical examination findings, and risk factors for HDV infection.

Performance characteristics have not been established for the following specimen characteristics:

• Grossly icteric (total bilirubin level of >20 mg/dL)
• Grossly lipemic (triolein level of >3000 mg/dL)
• Grossly hemolyzed (hemoglobin level of >500 mg/dL)
• Containing particulate matter
• Cadaveric specimens

References

1. Mayo Clinic Laboratories Clinical & Interpretive Information: Hepatitis D Virus Total Antibodies

Ordering & Collection

Specimen Type

Blood

Collection

Collect 3mL blood in RED TOP or GOLD SST tube

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial within 2 hours of collection. Freeze serum at -20°C while awaiting shipment. Transport samples on dry ice.

Stability: Frozen (preferred): 30 days; Refrigerated: 30 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Quantity

Requested: 1 mL serum
Minimum: 0.5 mL serum

Processing

Receiving Instructions

Centrifuge and transfer serum to a separate plastic vial within 2 hours of collection. Freeze serum at -20°C.

Sendouts:

• Order Mayo Test: AHDV.
• Interfaced: Yes.

Stability: Frozen (preferred): 30 days; Refrigerated: 30 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Misc Sendout

Performance

Lab Department

Sendouts Mayo Lab (FZ)(MAFZ)

Frequency

Performed: Varies. Maximum Laboratory Time: 7 days

Available STAT?

No

Performing Location(s)

Sendout	Mayo Clinic Laboratories 800-533-1710 200 First Street Southwest Rochester, MN 55901

Billing & Coding

CPT Codes

86692

LOINC

40727-0

Interfaced Order Code

UOW3967