Respiratory Virus PCR Panel (no SARS-CoV-2)

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General Information

Lab Name
Respiratory Virus PCR Panel (no SARS-CoV-2)
Lab Code
REVSQF
Epic Ordering
Respiratory Virus PCR Panel (no SARS-CoV-2)
Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and identification of multiple respiratory viral pathogens.

The following viruses are targeted (gene):

Adenovirus, AdV (Hexon)
human Metapneumovirus, hMPV (nucleocapsid)
Rhinovirus, RV (5” UTR)
Influenza A, FluA (matrix)
Influenza B, FluB (matrix)
Respiratory Syncytial Virus, RSV (matrix)
Human Parainfuenza virus type 1, hPIV1 (hemagglutinin neuraminidase)
Human Parainfuenza virus type 2, hPIV2 (hemagglutinin neuraminidase)
Human Parainfuenza virus type 4, hPIV3 (hemagglutinin neuraminidase)
Human Parainfuenza virus type 4, hPIV4 (hemagglutinin neuraminidase)
Coronavirus (CoV) subtypes OC43 (ORF1ab)
Coronavirus (CoV) subtypes HKU1 (ORF1ab)
Coronavirus (CoV) subtypes NL63 (Spike)
Coronavirus (CoV) subtypes 229R (ORF2ab)

Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.

The performance characteristics of the test were determined by the University of Washington Department of Laboratory Medicine and Pathology. The modified version has not been cleared or approved by the US Food and Drug Administration.

For ordering individual battery components:

FUSAMR, Adeno/Metapneumo/Rhino Virus PCR Panel [FUSAMR]

FLURSV, Influenza A, B & RSV PCR Panel [FLURSV]

FUSPIV, Parainfluenza PCR Panel [FUSPIV]

FUSCOV, Coronavirus (Non SARS CoV2) PCR Panel [FUSCOV]

Synonyms
Components
Code Name
FLURSV Influenza A/B & RSV by PCR
FLUSD Influenza A/B & RSV Specimen
FLAPCR Influenza A PCR Result
CTFLA Influenza A Ct Value
FLBPCR Influenza B PCR Result
CTFLB Influenza B Ct Value
RSVPCR RSV PCR Result
CTRSV RSV Ct Value
FLURI Influenza A/B & RSV Interp
FUSAMR Adeno/Metapneumo/Rhino Virus PCR Panel
FUSSD Adeno/Metapneumo/Rhino Virus PCR Spec
FUSADV Adenovirus Result
FUSACT Adenovirus Ct Value
FUSMPV Metapneumovirus Result
FUSNCT Metapneumovirus Ct Value
FUSRHV Rhinovirus Result
FUSRCT Rhinovirus Ct Value
FAMRIN Adeno/Metapneumo/Rhino Virus PCR Interp
FUSCOV Coronavirus (Non SARS CoV2) PCR Panel
FUCSD Coronavirus PCR Specimen
CVOC43 CoV OC43 Result
OC43CT CoV OC43 Ct Value
CVHKU1 CoV HKU1 Result
HKU1CT CoV HKU1 Ct Value
CVNL63 CoV NL63 Result
NL63CT CoV NL63 Ct Value
CV229E CoV 229E Result
229ECT CoV 229E Ct Value
FCOVIN Coronavirus PCR Interp
FUSPIV Parainfluenza PCR Panel
FUPSD Parainfluenza PCR Specimen
FUSPV1 Parainfluenza 1 Result
FPV1CT PIV1 Ct Value
FUSPV2 Parainfluenza 2 Result
FPV2CT PIV2 Ct Value
FUSPV3 Parainfluenza 3 Result
FPV3CT PIV3 Ct Value
FUSPV4 Parainfluenza 4 Result
FPV4CT PIV4 Ct Value
FPIVIN Parainfluenza PCR Interp

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Nasopharyngeal swab, Oropharyngeal swab, Anterior Nares swab, Broncho alveolar lavage (BAL), and tracheal aspirate.
Collection

Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.

Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.

Minimum sample requirement: 0.75 mL

Handling Instructions

Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.

Quantity
requested: 1.0 mL
minimum: 0.75 mL

Processing

Processing

Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Performing Location(s)
CL Virology, Renton
206-685-6656

1602 Raymond Ave SW

Renton, WA 98057-3356

206-685-6656 opt 4

Frequency
Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.
Available STAT?
No

Billing & Coding

CPT codes
87633
LOINC
81655-3
Interfaced Order Code
UOW4293