Parainfluenza PCR Panel

General Information

Lab Name

Lab Code

FUSPIV

Epic Name

Parainfluenza PCR Panel

Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and differentiation of Human parainfluenza viruses type 1, type 2 , type 3 and type 4 using real-time PCR.

The assays targets the hemagglutinin neuraminidase gene for each virus. Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.

Components

Code	Name
FUPSD	Parainfluenza PCR Specimen
FUSPV1	Parainfluenza 1 Result
FPV1CT	PIV1 Ct Value
FUSPV2	Parainfluenza 2 Result
FPV2CT	PIV2 Ct Value
FUSPV3	Parainfluenza 3 Result
FPV3CT	PIV3 Ct Value
FUSPV4	Parainfluenza 4 Result
FPV4CT	PIV4 Ct Value
FPIVIN	Parainfluenza PCR Interp

Interpretation

Guidelines

Guidelines for selecting respiratory virus testing

Method

Real-time Polymerase Chain Reaction.

Reference Range

See individual components

Ordering & Collection

Specimen Type: Nasopharyngeal swab, Oropharyngeal swab, Anterior Nares swab, Broncho alveolar lavage (BAL) and tracheal aspirate.
Collection: Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.

Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.

Minimum sample requirement: 0.75 mL
Handling Instructions: Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.
Quantity: Requested: 1 mL
Minimum: 750 µl

Processing

Receiving Instructions: Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.

Performance

Lab Department

Central Lab Virology(RVIR)

Frequency

Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.

Available STAT?

Performing Location(s)

CL	Virology, Renton 206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356	206-685-6656 opt 4

Billing & Coding

CPT Codes: 87631
LOINC: 81655-3
Interfaced Order Code: UOW4262