The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and differentiation of Human parainfluenza viruses type 1, type 2 , type 3 and type 4 using real-time PCR.
The assays targets the hemagglutinin neuraminidase gene for each virus. Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.
Code | Name |
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FUPSD | Parainfluenza PCR Specimen |
FUSPV1 | Parainfluenza 1 Result |
FPV1CT | PIV1 Ct Value |
FUSPV2 | Parainfluenza 2 Result |
FPV2CT | PIV2 Ct Value |
FUSPV3 | Parainfluenza 3 Result |
FPV3CT | PIV3 Ct Value |
FUSPV4 | Parainfluenza 4 Result |
FPV4CT | PIV4 Ct Value |
FPIVIN | Parainfluenza PCR Interp |
Real-time Polymerase Chain Reaction.
Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.
Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.
Minimum sample requirement: 0.75 mL
Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.
Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.
CL |
Virology, Renton
206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356 |
206-685-6656 opt 4 |
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