Coccidioides Antibody Initial Screen, Reflex to CF & ID (Sendout)

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General Information

Lab Name
Coccidiodies Ab Initial Screen, Reflex to CF & ID (Sendout)
Lab Code
RCOXIS
Epic Ordering
Coccidiodies Ab Initial Screen, Reflex to CF & ID (Sendout)
External Test Id
COXIS
Description

Useful For:

  • Detection of antibodies to Coccidioides immitis/posadasii
  • This assay should not be used for monitoring response to therapy.

Testing Algorithm: If result is positive, then Coccidioides by complement fixation and immunodiffusion will be performed at additional charge.

Ordering Guidance: Preferred initial Coccidioides antibody screening test. Refer to Coccidioides Antibody, for follow-up, CF & ID (Sendout) [RSCOCC] for monitoring of known positives.

References
Synonyms
Cocci serology, Coccidioides immitis, Coccidioides posadasii, COXIS, San Joaquin Valley Fever
Components

Interpretation

Method

Coccidioides Antibody Screen: Enzyme Immunoassay (EIA)

Reflex, if performed: Complement Fixation (CF) and Immunodiffusion (ID)

Reference Range
See individual components
Ref. Range Notes

Reference Values: Negative

Interpretation:

Enzyme immunoassay (EIA) results greater than or equal to 0.75 will be reported as Reactive: Confirmatory testing by complement fixation and immunodiffusion has been ordered.

A reactive result is presumptive evidence that the patient was previously or is currently infected with Coccidioides immitis/posadasii.

EIA results less than 0.75 will be reported as Negative: Repeat testing on a new sample in 2 – 3 weeks if clinically indicated.

A negative result indicates the absence of antibodies to Coccidioides immitis/posadasii and is presumptive evidence that the patient has not been previously exposed to and is not infected with Coccidioides. However, a negative result does not preclude the diagnosis of coccidioidomycosis as the specimen may have been drawn before antibodies levels were detectable due to early acute infection or immunosuppression.

This test is designed for the qualitative detection of both IgM- and IgG-class antibodies against antigens from Coccidioides. The report will not indicate which class of antibody is present.

Interferences and Limitations

Cautions:

All results from this assay must be correlated with clinical history, epidemiologic data, and other laboratory evidence.

Reactive results from this assay are not indicative of acute infection. Antibodies may be present from previous infection with Coccidioides immitis/posadasii.

Negative results may occur in patients with acute coccidioidomycosis in whom antibody levels have not yet become detectable.

Rarely, cross reactivity of the Coccidioides antibody screen may occur in patients infected with other dimorphic fungal agents including Histoplasma and Blastomyces. Therefore, all positive results must be confirmed by complement fixation and immunodiffusion.

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in GOLD SST or RED TOP tube

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia.

Quantity
requested: 2 mL serum
minimum: 1.7 mL serum

Processing

Processing

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Sendouts:

  • Order Mayo Test: COXIS.
  • Interfaced: Yes.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia.

Performance

LIS Dept Code
Sendouts Mayo Lab (RF) (MARF)
Performing Location(s)
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901

Frequency
Performed: Monday - Friday. Report Available: 1-7 days.
Available STAT?
No

Billing & Coding

CPT codes
86635
LOINC
40712-2
Interfaced Order Code
UOW4704