CD107a Mobilization (Sendout)

General Information

Lab Name

CD107a Mobilization (Sendout)

Lab Code

RCD107

Epic Name

Not individually orderable in Epic.

Order Hyperinflammatory Lab Panel.

External Test Id

2905400

Description

Test Utility: This test is ordered as part of the workup for hyperinflammatory syndromes such as hemophagocytic lymphohistiocytosis (HLH). This test should be ordered concurrently or after Hyperinflammatory Screening Labs have been obtained (CBC with diff, ferritin, fibrinogen, triglycerides, LDH, and D-dimer).

The following are consistent with, but not specific for, a diagnosis of HLH:

-Elevated sIL-2R, CXCL-9, and IL-18
-Elevated sCD163
-Reduced NK function (or cell surface expression of CD107alpha)
-Reduced perforin, SAP, or XIAP

Test Description: This employs flow cytometry to evaluate natural killer (NK) cell function/degranulation using surface expression of CD107alpha (also called LAMP-1 [lysosomal-associated membrane protein 1]). Mononuclear cells from the peripheral blood are stimulated with K562 target cells which induce degranulation. Tagged anti-CD107a is present with the cells during the stimulation period. After a 6-hour incubation at 37°C, the cells are surface-stained with NK cell markers (as well as CD3 and CD8). If the NK cells have been degranulated, CD107a will be expressed on the effector surface and will be detected by flow cytometry and reported as percent expression and mean channel fluorescence (MCF). Sample viability is assessed by flow cytometry using 7-AAD. Samples with a cellular viability of >=80% are reported. Samples with a cellular viability of 75-79% will be run and assessed for reportability.

Ordering Requirements: Laboratory Medicine Resident (LMR) approval is required for the Hyperinflammatory Lab Panel to help ensure that when this panel is indicated, collections are timed appropriately to meet stability limits for Perforin/Granzyme B (Sendout) [RPERGB] and CD107a Mobilization (Sendout) [RCD107].

Collection Note: Limited specimen stability. Samples must be received at Cincinnati Children’s Hospital Lab within 24 hours of collection, on days that align with Cincinnati Lab's run schedule. Time blood collections accordingly. Failure to follow collection guidelines may result in test cancellations.

***Note: Effective October 27, 2026, this test is offline at Cincinnati Children's Lab and has been replaced with CD107a Cytotoxic Lymphocyte Exocytosis Assay (Sendout) [#1206]. Results will be reported using "Reference Lab Test 1" [SEND1] until a new test code is available on 01/04/2027.***

Synonyms

2905400, CD107a NK cell, Hemophagocytic Lymphohistiocytosis, HLH, Hyperinflammatory disorder, NK cell CD107a expression, NK Cell Degranulation

Components

Code	Name
RCDPNK	CD107a Positive NK
RCDMCF	CD107a MCF
RCD17I	CD107a Interpretation
RCD17C	CD107a Comment