ABL Kinase Mutational Analysis

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General Information

Lab Name
ABL Kinase Mutational Analysis
Lab Code
RABLKM
Epic Ordering
ABL KINASE MUTATIONAL ANALYSIS
Description

ABL Kinase Mutational Analysis Assay is indicated for patients who have developed a clinical resistance to imatinib (Gleevec) therapy. Mutations in the ABL Kinase domain of the BCR/ABL oncogene have differing treatment options, depending upon the specific mutation. Through detection of mutations, alternate therapies may be warranted. Results are qualitative. The ABL Kinase Mutational Analysis can be applied to samples which have tested positive for the BCR/ABL transcript.

References

References:

  • Blood (2002); 99(9): 3472-3475
  • Blood (2003); 102(1): 276-283
  • Methods in Molecular Medicine (2006); 125: 93-106
  • Med Oncol (2012); 29(3): 2136-2142
  • Blood (2011); 118(19): 5250-5254
Forms & Requisitions

Fred Hutch Molecular Oncology Laboratory Requisition Form

Synonyms
Gleevec Resistance, Imatinib Resistance, T315I Mutation, TKI Resistance
Components

Interpretation

Method

RT-PCR

Reference Range
See individual components
Interferences and Limitations

Assay Considerations: This assay detects mutations in the ABL Kinase Domain, ranging from amino acids 235 to 4863. Allelic ratios are estimated for all positive results, however this assay is not considered quantitative. The limit of detection for most mutations is about 20%, but may be lower in some cases. It should be noted that stochastic variations may influence the sensitivity and precision of the assay, especially when levels of BCR/ABL transcripts are low.

References

References:

  • Blood (2002); 99(9): 3472-3475
  • Blood (2003); 102(1): 276-283
  • Methods in Molecular Medicine (2006); 125: 93-106
  • Med Oncol (2012); 29(3): 2136-2142
  • Blood (2011); 118(19): 5250-5254

Ordering & Collection

Specimen Type
Blood, Bone Marrow
Collection
  • Blood: 3 - 5 mL whole blood in LAVENDER Top (EDTA) tube
  • Bone Marrow: 1 - 3 mL bone marrow in LAVENDER Top (EDTA) tube

Recommended Collection: Sunday through Thursday.

  • If collecting on Friday, collect prior to 1 p.m. and expedite transport to the lab. Samples must be received at Fred Hutch Molecular Oncology Lab by 3 p.m. on Friday.

Outside Clients: Collect Sunday (afternoon) through Thursday only. Expedite transport (samples are stable for 48 hours).

Forms & Requisitions

Fred Hutch Molecular Oncology Laboratory Requisition Form

Handling Instructions

Transport sample to the laboratory immediately. Sample must be received by the Fred Hutch Molecular Oncology Laboratory within 48 hrs of collection.

Quantity
requested: 7 mL Peripheral Blood OR 3 mL Bone Marrow
minimum: 5 mL Peripheral Blood OR 1 mL Bone Marrow

Processing

Processing

Refrigerate whole blood or bone marrow. Notify Sendouts immediately upon receipt of sample.

Sendouts: Expedite handling.

  • Send samples Monday - Friday to Fred Hutch via Delivery Express courier.
  • Samples collected on Friday must be received by Fred Hutch Molecular Oncology Lab by 3 pm. Contact the lab at (206) 667-2592 prior to sending to ensure that samples will be processed.

Alliance lab: Promptly forward specimen to Fred Hutch Molecular Oncology Lab. Do not route sample to UW-MT. Forward a copy of the requisition to UW-MT Sendouts, noting that the sample has been sent directly.

Stability: Refrigerated: 48 hrs; Frozen: Unacceptable.

Performance

LIS Dept Code
Sendouts (SO)
Performing Location(s)
Sendout Fred Hutchinson Cancer Research Center, Molecular Oncology Laboratory
206-667-2592

1100 Fairview Ave N
D2-281
Seattle, WA 98109
Frequency
Turnaround Time: 3-5 business days.
Available STAT?
No

Billing & Coding

CPT codes
81170
LOINC
55135-8
Interfaced Order Code
UOW2909