Lower Respiratory Bacterial Culture and Sensitivity with Gram Stain

General Information

Lab Name
Lower Resp C/S w/Gram
Lab Code
LRSC
Epic Name
Culture Resp Lower, Bact w/ Stain
Description
Synonyms
BAL Culture, Bronchial Culture, ETT Culture, Sputum Culture
Components

Interpretation

Guidelines
Reference Range
See individual components

Ordering & Collection

Specimen Type
Sputum, Bronchial Wash, Tracheal Aspirate, Endotracheal Tube Suction (ETT), Bronchoalveolar lavage (BAL)
Collection

Sputum: obtain specimen from deep cough (usually in AM), induction or intubation; do not send saliva.

Sputum gram stains are screened for oropharyngeal contamination and specimens are rejected for culture if the gram stain shows few WBC's and moderate squamous epithelial cells. A new specimen will be requested for culture.

Handling Instructions

Transport specimens in a sterile, leak-proof container. Ideally, specimens should be transported to the laboratory within 2 hours of collection. If transport time will exceed 2 hours, specimens should be stored and transported refrigerated.

Specimens will be rejected if received > 24 hours after collection. Viability of organisms is significantly reduced after 24 hours.

Quantity
Requested: Sputum: 2-5 mL, Tracheal Aspirate: 1-2 mL, BAL: > 1 mL
Minimum: 1 mL

Processing

Receiving Instructions

If quantitative culture is requested on BAL (not sputum), order LRSQNC.

Misc Sendout

Performance

Lab Department
Microbiology(MC)
Frequency
Processed upon receipt
Available STAT?
Yes-Gram stain
Performing Location(s)
HMC Microbiology
206-744-5858

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

UW-MT Microbiology
206-520-4600

Clinical Lab, Room # NW177,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Other Locations/Notes

UW-MT Microbiology performs testing for UW-MT and UW-NW.

Billing & Coding

CPT Codes
87070, 87205
LOINC
6460-0
Interfaced Order Code
UOW2679
Billing Comments

Bronchial fluid Gram stains are concentrated by cytocentrifugation, which is an additional charge (see ADDCON).