HSV Acyclovir Resistance with Rapid PCR, Swab/CSF
General Information
- Lab Name
- HSV Acyclovir Resistance with Rapid PCR, Swab/CSF
- Lab Code
- HSVDRR
- Epic Name
- HSV Acyclovir Resistance with Rapid PCR, Swab/CSF
- Description
HSV Semi-Quantitative Rapid PCR, Swab/CSF, performed using the DiaSorin Simplexa™ HSV-1 & HSV-2 Direct FDA-approved assay HSV SemiQnt Rapid PCR, Swab/CSF [RPDHSV].
For positive specimens with a PCR Cycle Threshold (Ct) value <35.0, HSV Acyclovir Drug Resistance testing is reflexively performed. The HSV Acyclovir Drug Resistance assay detects and characterizes resistance-associated mutations in the Thymidine Kinase (UL23) gene of HSV-1 and HSV-2.
Note: The HSV Acyclovir Drug Resistance HSV Acyclovir Drug Resistance by Sanger Sequencing [HSVDR] assay is no longer orderable as a standalone test. To add HSVDR to a previous RPDHSV, use LADDON for SPS addon. LADDON HSVDR only to the following resulted test:
- HSV SemiQnt Rapid PCR, Swab/CSF [RPDHSV] (CSF/swabs), if CTHSV1 or CTHSV2 Ct value is <35.0
- HSV SemiQnt Rapid PCR, Swab/CSF [RPDHSV] (CSF/swabs), if CTHSV1 or CTHSV2 Ct value is <35.0
- Synonyms
- Acyclovir, Drug Resistance, Herpes Simplex Virus by PCR, HSV, HSV DNA, HSV Qualitative PCR, PCR for Herpes Simplex, Sanger
- Components
-
Code Name RHSVSD HSV Rapid PCR Specimen Description RHSV1 HSV 1 Rapid PCR Result CTHSV1 HSV 1 Rapid CT Value RHSV2 HSV 2 Rapid PCR Result CTHSV2 HSV 2 Rapid CT Value RHSVIN HSV Rapid PCR Interpretation HSVDRS HSV Drug Resistance Status
Interpretation
- Method
This test was developed, and its performance characteristics were determined, by the University of Washington Department of Laboratory Medicine and Pathology. It is important to note that while this test has been validated for clinical use, it has not been cleared or approved by the US Food and Drug Administration (FDA). However, under the Clinical Laboratory Improvement Amendments (CLIA), the University of Washington laboratory ensures the test meets high standards for accuracy and reliability in a clinical setting.
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Swabs, CSF
- Collection
-
Preferred specimen: Swabs, CSF
Unacceptable specimens: Heparin containing specimens.
- Quantity
-
Requested: 0.75 mL
Minimum: 0.5 mL
Processing
- Receiving Instructions
HSVDRR specimen processing:
- Swabs: Transport specimens on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
- CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Transport CSF on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
Outside Laboratory: Ship samples on dry ice. If -60 to -85°C freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 7 days of collection. Samples stored at 2-8°C greater than 7 days old may be rejected. Do not freeze/thaw.
- Swabs: Transport specimens on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
- Misc Sendout
Performance
- Lab Department
- Clinical Virology(CVIR)
- Frequency
- Rapid PCR is performed daily except Sunday. Sequencing is performed Wednesday.
- Available STAT?
- No
- Performing Location(s)
-
Other UW Medicine Central Laboratory 1601 Lind Ave SW
Renton, WA 98057Phone: 206-685-6656 option 8
Hours of Operation:
Mon–Fri: 7:00 AM–7:00 PM
Sat: 9:00 AM–4:00 PM
Billing & Coding
- CPT Codes
- 87529x2
- LOINC
- 29578-2
- Interfaced Order Code
- UOW6441