HSV SemiQnt Rapid PCR, Swab/CSF
General Information
- Lab Name
- HSV SemiQnt Rapid PCR, Swab/CSF
- Lab Code
- RPDHSV
- Epic Ordering
- HSV Semiqnt Rapid PCR, Swab/CSF
- Description
The DiaSorin Simplexa™ HSV 1 & 2 Direct FDA approved assay is a rapid real-time PCR that enables the direct amplification, detection and differentiation of HSV-1 and/or HSV-2 DNA from both cutaneous and mucocutaneous swab specimens and CSF specimens. Well conserved regions of the HSV-1 and HSV-2 DNA polymerase genes are targeted to identify HSV-1 and HSV-2 DNA respectively in the specimen. Results are reported as "detected" or "not detected" and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.
For Blood or Clear non-cellular fluid (e.g. BAL, or Washing), see HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid [HS12QN].
For Tissue Biopsy, Bone Marrow or Cellular fluid (e.g. BAL or Washing), see HSV Type 1 & 2 Qual PCR, Tissue [HS12QL].
- Synonyms
- HSV DNA, HSV Qualitative PCR
- Components
-
Code Name RHSVSD Rapid HSV PCR Specimen Desc. RHSV1 Rapid HSV 1 PCR Result CTHSV1 Rapid HSV1 CT Value RHSV2 Rapid HSV 2 PCR Result CTHSV2 Rapid HSV2 CT Value RHSVIN Rapid HSV PCR Interpretation
Interpretation
- Method
Real-time Polymerase Chain Reaction.
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Swab or CSF
- Collection
-
Swab: Acceptable specimen types are cutaneous and mucocutaneous swabs stored in UTM, Remel M4, Remel M4RT, Remel M5, or Remel M6 transport media. Follow the manufacturers’ package inserts for collection media and acceptable swab types. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing.
CSF: 1 mL in sterile container
Unacceptable samples: Calcium alginate swabs, as they may contain substances that inhibit PCR testing.
Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.
Minimum sample requirement: 0.1 mL
- Handling Instructions
- Quantity
-
requested: 1 mL
minimum: 0.1 mL
Processing
- Processing
Swabs: Transport specimens on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Transport CSF on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
Outside Laboratory: Ship samples on dry ice. If -60 to -85°C freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 7 days of collection. Samples stored at 2-8°C greater than 7 days old may be rejected. Do not freeze/thaw.
Performance
- LIS Dept Code
- Clinical Virology (CVIR)
- Performing Location(s)
-
Other Virology
206-685-8037Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102 - Frequency
- Monday- Friday 07:00- 21:00 and Saturday 09:00-16:00
- Available STAT?
- Needs Laboratory Medicine Resident approval
Billing & Coding
- CPT codes
- 87529x2
- LOINC
- 32141-4
- Interfaced Order Code
- UOW3266