HSV Acyclovir Resistance with Quant PCR, Blood/Eye Fluid

General Information

Lab Name

Lab Code

HSVDRQ

Epic Name

HSV Acyclovir Resistance with Quant PCR, Blood/Eye Fluid

Description

The detection and quantitation of Herpes Simplex virus by real-time PCR using the HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid [HS12QN] assay.

For positive specimens with >1000 copies/mL, HSV Acyclovir Drug Resistance testing is reflexively performed. The HSV Acyclovir Drug Resistance assay detects and characterizes resistance-associated mutations in the Thymidine Kinase (UL23) gene of HSV-1 and HSV-2.

Note: The HSV Acyclovir Drug Resistance HSV Acyclovir Drug Resistance by Sanger Sequencing [HSVDR] test is no longer orderable as a standalone test. To add HSVDR to a previous HS12QN, use LADDON for SPS addon. LADDON HSVDR only to the following resulted test:

HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid [HS12QN] (blood/body fluids): If HSVQN1 or HSVQN2 is positive ≥ 1000 copies/mL

Synonyms

Acyclovir, Drug Resistance, Herpes Simplex Virus by PCR, HSV, HSV DNA, HSV Quantitative PCR, PCR for Herpes Simplex, Sanger

Components

Code	Name
HSVQNS	HSV Quantitative PCR Specimen Description
HSVQN1	HSV 1 Quantitative PCR Result
HSVLO1	HSV 1 Quantitative PCR Result (Log 10)
HSVQN2	HSV 2 Quantitative PCR Result
HSVLO2	HSV 2 Quantitative PCR Result (Log 10)
HSVQNI	HSV Quantitative PCR Interpretation
HSVQNM	HSV Quantitative PCR Method
HSVDRS	HSV Drug Resistance Status

Interpretation

Method: This test was developed, and its performance characteristics were determined, by the University of Washington Department of Laboratory Medicine and Pathology. It is important to note that while this test has been validated for clinical use, it has not been cleared or approved by the US Food and Drug Administration (FDA). However, under the Clinical Laboratory Improvement Amendments (CLIA), the University of Washington laboratory ensures the test meets high standards for accuracy and reliability in a clinical setting.
Reference Range: See individual components

Ordering & Collection

Specimen Type: Blood or Clear non-cellular fluid (e.g. BAL, Washing).
Collection: Preferred specimen: Blood or Clear non-cellular fluid (e.g. BAL, Washing).

Unacceptable specimens: Heparin containing specimens
Quantity: Requested: 1.0 mL
Minimum: 0.75 mL

Processing

Receiving Instructions

HSVDRQ specimen processing:

Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Eye Fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Clear non-cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Misc Sendout

Performance

Lab Department

Central Lab Virology(RVIR)

Frequency

Quantitative PCR is performed Tuesday and Friday. Sequencing is performed Wednesday.

Available STAT?

Performing Location(s)

Other	UW Medicine Central Laboratory 1601 Lind Ave SW Renton, WA 98057	Phone: 206-685-6656 option 8 Hours of Operation: Mon–Fri: 7:00 AM–7:00 PM Sat: 9:00 AM–4:00 PM

Billing & Coding

CPT Codes: 87530x2
LOINC: 29578-2
Interfaced Order Code: UOW6443