HSV Acyclovir Resistance with Quant PCR, Blood/Eye Fluid

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General Information

Lab Name
HSV Acyclovir Resistance with Quant PCR, Blood/Eye Fluid
Lab Code
HSVDRQ
Epic Name
HSV Acyclovir Resistance with Quant PCR, Blood/Eye Fluid
Description

The detection and quantitation of Herpes Simplex virus by real-time PCR using the HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid [HS12QN] assay.

For positive specimens with >1000 copies/mL, HSV Acyclovir Drug Resistance testing is reflexively performed. The HSV Acyclovir Drug Resistance assay detects and characterizes resistance-associated mutations in the Thymidine Kinase (UL23) gene of HSV-1 and HSV-2.

Note: The HSV Acyclovir Drug Resistance HSV Acyclovir Drug Resistance by Sanger Sequencing [HSVDR] test is no longer orderable as a standalone test. To add HSVDR to a previous HS12QN, use LADDON for SPS addon. LADDON HSVDR only to the following resulted test:

Synonyms
Acyclovir, Drug Resistance, Herpes Simplex Virus by PCR, HSV, HSV DNA, HSV Quantitative PCR, PCR for Herpes Simplex, Sanger
Components
Code Name
HSVQNS HSV Quantitative PCR Specimen Description
HSVQN1 HSV 1 Quantitative PCR Result
HSVLO1 HSV 1 Quantitative PCR Result (Log 10)
HSVQN2 HSV 2 Quantitative PCR Result
HSVLO2 HSV 2 Quantitative PCR Result (Log 10)
HSVQNI HSV Quantitative PCR Interpretation
HSVQNM HSV Quantitative PCR Method
HSVDRS HSV Drug Resistance Status

Interpretation

Method

This test was developed, and its performance characteristics were determined, by the University of Washington Department of Laboratory Medicine and Pathology. It is important to note that while this test has been validated for clinical use, it has not been cleared or approved by the US Food and Drug Administration (FDA). However, under the Clinical Laboratory Improvement Amendments (CLIA), the University of Washington laboratory ensures the test meets high standards for accuracy and reliability in a clinical setting.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood or Clear non-cellular fluid (e.g. BAL, Washing).
Collection

Preferred specimen: Blood or Clear non-cellular fluid (e.g. BAL, Washing).

Unacceptable specimens: Heparin containing specimens

Quantity
Requested: 1.0 mL
Minimum: 0.75 mL

Processing

Receiving Instructions

HSVDRQ specimen processing:

  • Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

  • Eye Fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

  • Clear non-cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Misc Sendout

Performance

Lab Department
Central Lab Virology(RVIR)
Frequency
Quantitative PCR is performed Tuesday and Friday. Sequencing is performed Wednesday.
Available STAT?
No
Performing Location(s)
Other UW Medicine Central Laboratory

1601 Lind Ave SW
Renton, WA 98057

Phone: 206-685-6656 option 8
Hours of Operation:
Mon–Fri: 7:00 AM–7:00 PM
Sat: 9:00 AM–4:00 PM

Billing & Coding

CPT Codes
87530x2
LOINC
29578-2
Interfaced Order Code
UOW6443