Human Herpes Virus Type 8 by PCR Qualitative Tissue or Cells

General Information

Lab Name

HHV8 PCR Qualitative

Lab Code

HH8QLT

Epic Name

HHV8 PCR Qualitative

Description

The detection of Human Herpesvirus (HHV-8), also known as Kaposi’s sarcoma associated herpesvirus (HHV-8, KSHV), by real-time PCR amplification. The primers used in this PCR reaction amplify the ORF 73 gene. Detection of HHV-8 DNA (copies/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the HHV-8 amplicons. The minimum virus level that gives a positive result in 95% of replicates is 500 copies/mL (2.70 log copies/mL). Results are reported as “positive” or “not detected”.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF, or Clear non-cellular fluid (e.g. BAL, or Washing), see HHV8 PCR, Quantitative [HH8QN].

Synonyms

HHV-8, HHV8 PCR Qualitative

Components

Code	Name
HH8QLS	HH8 Qual Specimen Descrip
HH8QLR	HH8 Qual Result
HH8QLI	HH8 Qual Interp

Interpretation

Guidelines

Washington DOH Notifiable Conditions

Method

Real-time Polymerase Chain Reaction.

Reference Range

See individual components

Ordering & Collection

Specimen Type: Tissue Biopsy, Paraffin Tissue, Bone Marrow, Swab or Cellular fluid (e.g. BAL or Washing)
Collection: Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).

Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube.

Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube. No Heparin aspirate in syringe.

Swab: In sterile tube/universal transport media (UTM).

Cellular fluid: 1 mL in sterile tube.

Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.

Minimum sample requirement: 0.5 mL
Handling Instructions: Bone Marrow: Send LAVENDER TOP to Laboratory at room temperature within 24 hour of collection.

Note: Separate specimens MUST be submitted when both Viral Culture and PCR testing are ordered.
Quantity: Requested: See Collection
Minimum: 0.5 mL

Processing

Receiving Instructions: Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Paraffin Tissue: Ship at room temperature.

Bone Marrow: Ship at room temperature within 24 hours of collection.

Swabs: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory:

Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder and ship on dry ice.

Tissue/Cellular fluid/Swab: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Misc Sendout

Performance

Lab Department

Virology, Eastlake(EVIR)

Frequency

Extraction Wednesday. PCR and results following Thursday.

Available STAT?

Performing Location(s)

Other	Virology, Molecular 206-685-6656 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Billing & Coding

CPT Codes: 87798
LOINC: 32364-2
Interfaced Order Code: UOW1341