Hepatitis B Surface Antigen & Antibody (HBsAg, HBsAb)

General Information

Lab Name

Hepatitis B Surface Ag and Ab

Lab Code

HBSS

Epic Name

Hepatitis B Surface Antigen and Antibody

Description

A Battery of tests for Hepatitis B virus markers in human sera by the FDA approved Abbott Architect test method:

Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb). Results are reported as mIU/mL.
Qualitative detection of Hepatitis B virus Surface Antigen (HBSAg).

Reactive HBSAg tests are confirmed by neutralization assay at no charge. Confirmed positives are reflexed to Hepatitis B Quantitative [HBVQNT] for final confirmation with an additional charge.

For HBSAg testing without reflex testing, see Hepatitis B Surface Antigen without PCR reflex for REACTIVE [HBSAGX].

For ordering individual battery components:

HBSAb, see Hepatitis B Surface Antibody (HBsAb) [HBSA].

HBSAg, see Hepatitis B Surface Antigen (HBsAg) with reflex to PCR for REACTIVE [HBSAG].

Synonyms

HBV, Hepatitis B Vaccine Screen

Components

Code	Name
HBAG	Hepatitis B Surface Antigen w/Reflex
BSAB	Hepatitis B Surface Ab
IUAB	Hepatitis B Surf Ab Intl Units
IN34	Hepatitis B Surf Ag and Ab Interp

Interpretation

Guidelines

Washington DOH Notifiable Conditions

Method

Chemiluminescence Immunoassay

Reference Range

See individual components

Ref. Range Notes

Per manufacture’s package insert protective level of HBSAb is ≥12 mIU/mL.

Ordering & Collection

Specimen Type: Blood
Collection: All Locations: 2 tubes required if reflex testing is ordered

Preferred: 5 mL blood in GOLD SST tube plus 5 mL blood in PEARL PPT tube

Also acceptable: 5 mL blood in ORANGE RST or RED TOP tube plus a 5 mL PEARL PPT tube for reflexive testing.

Note: If no PEARL PPT is received, any reflexive molecular testing can be done on GOLD SST, LAVENDER or RED TOP Tube that has not been on automated lines.
Quantity: Requested: 1.0 mL serum
Minimum: 0.5 mL serum

Processing

Receiving Instructions: GOLD SST tube: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.

PEARL PPT tube: centrifuge within 6 hours of collection and freeze spun PEARL PPT tube at -20°C. Route frozen PEARL tubes to UW SPS -20°C freezer rack 80. [ok to freeze spun pearl top tubes on gel.]

Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination or are grossly hemolyzed.

Misc Sendout

Performance

Lab Department

Central Lab Virology(RVIR)

Frequency

Monday-Friday

Available STAT?

Performing Location(s)

CL	Virology, Renton 206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356	206-685-6656 opt 4

Billing & Coding

CPT Codes: 86706, 87340
LOINC: 18719-5
Interfaced Order Code: UOW4733