Hepatitis B Surface Antibody (HBsAb)
General Information
- Lab Name
- Hepatitis B Surface Ab
- Lab Code
- HBSA
- Epic Name
- Hepatitis B Surface Antibody
- Description
The Quantitative detection Hepatitis B virus Surface IgG antibody (HBSAb) in human sera using the FDA approved Abbott ARCHITECT AUSAB-DIL test two-step chemiluminescent immunoassay.
In the first step, sample, assay diluent, and recombinant Hepatitis B surface Antigen (rHBsAg) coated paramagnetic microparticles are combined. Anti-HBs present in the sample binds to the rHBsAg coated microparticles. In the second step, rHBsAg acridinium-labeled conjugate is added, which binds to IgG anti-HBs. Then pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units (RLUs).
A direct relationship exists between the amount of anti-HBs in the sample and the RLUs. The concentration of anti-HBs in the sample is determined using an active ARCHITECT AUSAB calibration curve. Results are reported as mIU/mL.
For Batteries containing HBSAb see:
Hepatitis B Antibodies (HBcAB, HBsAB) [HBAB], Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb) and Hepatitis B virus Core IgG and IgM antibodies (HBCAb)
Hepatitis B Battery [HBB], Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb), Qualitative detection of Hepatitis B virus Surface Antigen (HBSAg) and Qualitative detection of Hepatitis B virus Core IgG and IgM antibodies (HBCAb)
Hepatitis B Surface Antigen & Antibody (HBsAg, HBsAb) [HBSS], Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb) and Qualitative detection of Hepatitis B virus Surface Antigen (HBSAg)
- Synonyms
- HBsAB-IgG, Hepatitis Titer
- Components
-
Code Name BSAB Hepatitis B Surface Ab IUAB Hepatitis B Surf Ab Intl Units IN7 Hepatitis B Surface Ab Interp
Interpretation
- Method
Chemiluminescence Immunoassay
- Reference Range
- See individual components
- Ref. Range Notes
Per manufacture’s package insert protective level is ≥12 mIU/mL.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Blood: 5 mL blood in GOLD TOP serum separator tube (SST).
Also acceptable: 5 mL blood in ORANGE TOP rapid serum tube (RST) or RED TOP tube
- Quantity
-
Requested: 1 mL serum
Minimum: 0.5 mL serum
Processing
- Receiving Instructions
Blood: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.
Outside Laboratories: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.
Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination or are grossly hemolyzed.
- Misc Sendout
Performance
- Lab Department
- Central Lab Virology(RVIR)
- Frequency
- Monday-Friday
- Available STAT?
- No
- Performing Location(s)
-
CL Virology, Renton
206-685-66561602 Raymond Ave SW
Renton, WA 98057-3356
206-685-6656 opt 4
Billing & Coding
- CPT Codes
- 86706
- LOINC
- 10900-9
- Interfaced Order Code
- UOW110