FLT3 TKD D835/I836 Mutations

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General Information

Lab Name
FLT3 TKD D835/I836 Mutations
Lab Code
FLTTKD
Epic Ordering
FLT3-TKD D835/I836 Mutations
Description

This assay detects mutations in the FLT3 gene at codons D835/I836 of the tyrosine kinase domain (FLT3 TKD). Evaluation for FLT3 point mutations in the TKD is indicated at diagnosis of acute myeloid leukemia and may provide prognostic information and direct treatment. Positive FLT3 TKD results may be further evaluated by next-generation sequencing to identify the specific mutation upon request. This is a qualitative and assay does not detect other point mutations throughout the TKD nor the FLT3 internal tandem duplication (FLT3-ITD).

Limit of Detection: 2.5%

Hematopathology test request form found at Request Form

Forms & Requisitions

Requisition

Synonyms
FLT3 D835 and I836, FLT3 D835/I836, FLT3 point mutation, FLT3 TKD, FLT3 tyrosine kinase domain, Heme Amplicon Panel by NGS (HOTSPT), Heme Single Gene by NGS (HOTSGH), UW Oncoplex Cancer Gene Panel (OPX)
Components

Interpretation

Method

PCR amplification of DNA followed by restriction enzyme and capillary electrophoresis for size fractionation

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Blood, Bone Marrow, Tissue - Fresh, frozen or FFPE/PET (Blocks), Fluids, Slides, Sorted Cells
Collection

Preferred:
Blood: 6mL in Lavender Top (EDTA)

Bone Marrow: 1-2mL in Lavender Top (EDTA)

Tissue, Fluids or Sorted cells: Sterile container in RPMI

Formalin-fixed paraffin embedded tissue (FFPE/PET) block

Slides: 10 unstained

Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.

Extracted DNA from CLIA certified laboratory.

Formalin-fixed paraffin embedded tissue (FFPE/PET)

Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides


Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives

Forms & Requisitions

Requisition

Handling Instructions
  • Deliver samples to Hematopathology as soon as possible.
  • If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
  • Samples stored at room temperature or refrigerated older than 7 days will be rejected.
  • Frozen samples should be shipped on dry ice.
Quantity
requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides

Processing

Processing

Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.

Performance

LIS Dept Code
Molecular Hematopathology (MHP)
Performing Location(s)
Other Hematopathology
206-606-7060

UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109

Hematopathology hours:
5:30 am - 6:30 pm Monday through Saturday
7:30 am - 4:00 pm Sunday, and with reduced staffing on major holidays
Other Hematopathology, Molecular
206-606-7060

UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109

Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays
Frequency
Monday, Wednesday, Friday: results available within 24hrs. EVALHD reflexed or add-on orders may take up to 5 days to complete.
Available STAT?
No

Billing & Coding

CPT codes
81246
LOINC
72520-0
Interfaced Order Code
UOW3325