FLT3 TKD D835/I836 Mutations

This assay detects mutations in the FLT3 gene at codons D835/I836 of the tyrosine kinase domain (FLT3 TKD). Evaluation for FLT3 point mutations in the TKD is indicated at diagnosis of acute myeloid leukemia and may provide prognostic information and direct treatment. Positive FLT3 TKD results may be further evaluated by next-generation sequencing to identify the specific mutation upon request. This is a qualitative and assay does not detect other point mutations throughout the TKD nor the FLT3 internal tandem duplication (FLT3-ITD).

Limit of Detection: 2.5%

Hematopathology test request form found at Request Form

Preferred:
Blood: 6mL in Lavender Top (EDTA)

Bone Marrow: 1-2mL in Lavender Top (EDTA)

Tissue, Fluids or Sorted cells: Sterile container in RPMI

Formalin-fixed paraffin embedded tissue (FFPE/PET) block

Slides: 10 unstained

Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.

Extracted DNA from CLIA certified laboratory.

Formalin-fixed paraffin embedded tissue (FFPE/PET)

Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides

Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives