BK Virus by PCR Quantitative

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General Information

Lab Name
BK Virus PCR Quant
Lab Code
BKVQN
Epic Ordering
BK Virus PCR Quant
Description

Blood (EDTA Plasma)

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® BKV Quantitative assay for the detection of BK polyomavirus (BKV) using real-time PCR amplification.

The lower limit of detection, LoD (the minimum virus level that gives a positive result in 95% of replicates), and the lower limit of quantitation, LLoQ, are both 21.5 IU/mL.

A conversion factor of 1.1 Log10 IU/mL is equivalent to 1.0 Log10 copies/mL.

The Roche cobas® BKV assay uses primers specific for the BKV VP2 region and the BKV small t-antigen region.

For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g. BAL or Washing), see BK Virus by PCR Qualitative [BKVQL].

For Urine, see BK Virus by PCR Quantitative, Urine [UBKQN].

Synonyms
Polyomavirus Type BK-Quant
Components

Interpretation

Method

Real-time Polymerase Chain Reaction

Reference Range
See individual components
Ref. Range Notes

Not Applicable

Ordering & Collection

Specimen Type
Blood
Collection

Blood: 3 mL EDTA plasma or PPT

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Serology and PCR testing are ordered.

Handling Instructions

Freshly drawn whole blood specimens may be held at 2 to 25°C for up to 24 hours prior to plasma preparation through centrifugation.

Quantity
requested: 1.0 mL of EDTA plasma
minimum: 350µL of EDTA plasma

Processing

Processing

Blood: Centrifuge upon arrival, remove plasma from cells.

Plasma specimens may be stored and/or transported:

  • 2 to 30°C for up to 24 hours
  • 2 to 8°C for up to 6 days
  • -15°C to -80°C for long-term storage up to 6 months.

Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is unavailable, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 6 days of collection. Do not freeze/thaw.

Performance

LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
CL Virology, Renton
206-685-6656

1602 Raymond Ave SW

Renton, WA 98057-3356

206-685-6656 opt 4
Frequency
Tuesday and Friday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
87799
LOINC
32284-2
Interfaced Order Code
UOW274