BK Virus by PCR Quantitative, Urine

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General Information

Lab Name
BK Virus PCR Quant, Urine
Lab Code
UBKQN
Epic Ordering
BK Virus PCR Quant, Urine
Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® BKV Quantitative assay for the detection of BK polyomavirus (BKV) using real-time PCR amplification.

The lower limit of detection, LoD (the minimum virus level that gives a positive result in 95% of replicates), is 12.2 IU/mL, and the lower limit of quantitation (LLoQ) is 200 IU/mL.

A conversion factor of 1.0 Log10 IU/mL is equivalent to 1.0 Log10 copies/mL.

The Roche cobas® BKV assay uses primers specific for the BKV VP2 region and the BKV small t-antigen region.

For Blood see BK Virus by PCR Quantitative [BKVQN].

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (e.g. BAL or Washing), see BK Virus by PCR Qualitative [BKVQL].

Synonyms
Polyomavirus Type BK-Urine Quant
Components

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Urine
Collection

Sterile Container. NO PRESERVATIVES.

Handling Instructions

Freshly collected urine specimens may be held at 2°C to 30°C for up to 24 hours or at 2°C to 8°C for up to 72 hours.

Unacceptable Samples: Urine with Preservative.

Quantity
requested: 10 mL urine
minimum: 1 mL urine

Processing

Processing

Send original urine container to Virology.

Urine specimen must be transferred into the cobas® PCR Media immediately upon arrival to the Virology lab.

  • 2 to 30°C for up to 90 days IN COBAS® PCR MEDIA

Performance

LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
CL Virology, Renton
206-685-6656

1602 Raymond Ave SW

Renton, WA 98057-3356

206-685-6656 opt 4
Frequency
Tuesday and Friday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
87799
LOINC
32284-2
Interfaced Order Code
UOW296