ANCA Reflexive Panel
General Information
- Lab Name
- ANCA Reflexive Panel
- Lab Code
- ANCAR
- Description
For neutrophil surface antibodies, see information under Lab Mnemonic: 102.
Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.
Anti PR3 and anti MPO [ANCARF] will be performed at an additional charge if the ANCA IFA is positive or if there is ANA staining that interferes with the detection of ANCA.
- Synonyms
- ANCAG, MPO, neutrophil cytoplasmic antibody, PR3, vasculitis, Wegeners
- Components
-
Code Name ANCAIF Antineutrophil Cytoplasmic Antibody by IF
Interpretation
- Method
Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.
- Reference Range
- See individual components
- Ref. Range Notes
Established by testing healthy blood donors during method validation.
- Interferences and Limitations
Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
6 mL blood in Red Top or GOLD SST tube
- Quantity
-
Requested: 2 mL serum
Minimum: 1 mL serum
Processing
- Receiving Instructions
Separate serum and refrigerate. Stable 14 days refrigerated. Stable long term frozen.
- Misc Sendout
Performance
- Lab Department
- Immunology(IMM)
- Frequency
- Monday-Friday
- Available STAT?
- No. See ANCAP or PRFAIL for STAT options
- Performing Location(s)
-
UW-MT Immunology
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Billing & Coding
- CPT Codes
- 86036
- LOINC
- 35279-9
- Interfaced Order Code
- UOW2949