Anti Neutrophil Cytoplasmic Antibody

General Information

Lab Name: Antineutrophil Cytoplasmic Antibody by IF
Lab Code: ANCAIF
Epic Ordering: Antineutrophil Cytoplasmic Antibody by IF
Description: For neutrophil surface antibodies, see information under Lab Mnemonic: 102

Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.
Synonyms: ANCA by IF, ANCA by Immunofluorescence

Method

Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.

Reference Range

Female		Male
Age	Range	Age	Range
0-	Negative [NRN]	0-	Negative [NRN]

Effective date: 04/01/2010

Ref. Range Notes

Established by testing healthy blood donors during method validation.

Interferences and Limitations

Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.

Processing: Refrigerate serum. Stable 14 days refrigerated. Stable long term frozen.

SPS, Do NOT use this mnemonic UNLESS the request specifies 'ANCA by IF or ANCA by Immunofluorescence'.

See: ANCAP for regular logins of ANCA's.

LIS Dept Code

Immunology (IMM)

Performing Location(s)

UW-MT	Immunology 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Monday-Friday

Available STAT?

See ANCAP or PRFAIL for STAT options