Anti Neutrophil Cytoplasmic Antibody

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General Information

Lab Name
Antineutrophil Cytoplasmic Antibody by IF
Lab Code
ANCAIF
Epic Ordering
Antineutrophil Cytoplasmic Antibody by IF
Description

For neutrophil surface antibodies, see information under Lab Mnemonic: 102

Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.

Synonyms
ANCA by IF, ANCA by Immunofluorescence

Interpretation

Method

Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.

Reference Range
Female Male
AgeRangeAgeRange
0-Negative [NRN] 0-Negative [NRN]

Effective date: 04/01/2010

Ref. Range Notes

Established by testing healthy blood donors during method validation.

Interferences and Limitations

Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in RED TOP or SST tube

Handling Instructions
Quantity
requested: 1 mL serum
minimum: 0.5 mL serum

Processing

Processing

Refrigerate serum. Stable 14 days refrigerated. Stable long term frozen.

SPS, Do NOT use this mnemonic UNLESS the request specifies 'ANCA by IF or ANCA by Immunofluorescence'.

See: ANCAP for regular logins of ANCA's.

Performance

LIS Dept Code
Immunology (IMM)
Performing Location(s)
UW-MT Immunology
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Frequency
Monday-Friday
Available STAT?
See ANCAP or PRFAIL for STAT options

Billing & Coding

CPT codes
86036
LOINC
35279-9
Interfaced Order Code
UOW691
Interfaced Result Code
UOW691