Anti Neutrophil Cytoplasm Panel

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General Information

Lab Name
Anti Neutrophil Cytoplasm Panel
Lab Code
ANCAP
Epic Ordering
Anti Neutrophil Cytoplasm Panel
Description

For neutrophil surface antibodies, see information under Lab Mnemonic: 102.

Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.

Synonyms
ANCA-Panel, ANCAG, Atypical ANCA, C ANCA, MPO, P ANCA, PR3, Vasculitis, Wegener's Antigen
Components

Interpretation

Method

Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.

Reference Range
See individual components
Ref. Range Notes

Established by testing healthy blood donors during method validation.

Interferences and Limitations

Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in Red Top or GOLD SST tube

Handling Instructions

Note: If requested STAT the ordering physician MUST call the Laboratory Medicine Resident on-call for approval.

Quantity
requested: 2 mL serum
minimum: 1 mL serum

Processing

Processing

Separate serum and refrigerate. Stable 14 days refrigerated. Stable long term frozen.

Performance

LIS Dept Code
Immunology (IMM)
Performing Location(s)
UW-MT Immunology
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Frequency
Monday-Friday
Available STAT?
Lab Medicine Resident approval required.

Billing & Coding

CPT codes
83520x2, 86036
LOINC
18719-5
Interfaced Order Code
UOW1551