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Bone Marrow Fungal Culture (Sendout)

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General Information

Lab Name
Lab Code
1098
External Test Id
FGEN
Description

Useful For: Diagnosing fungi as agent of infection in bone marrow.

Test Algorithm: If no growth after 24 days of incubation, a final negative report will be issued. If positive, organism identification will be performed at additional charge.

References
Synonyms
BNMF, Bone Marrow Fungal Culture, Bone Marrow Yeast Culture, FGEN, Fungal, Fungi, Fungus, Histoplasma, Histoplasmosis, Mold, Yeast
Components

Interpretation

Guidelines
Method

Method Description: Specimens are cultured on selective fungal media (e.g., inhibitory mold agar and brain heart infusion blood agar with chloramphenicol and gentamicin). Respiratory sources also are cultured on brain heart infusion agar with chloramphenicol, gentamicin, and cycloheximide. Cultures are incubated for 24 days at 30 degrees C.

Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, laboratory-developed real-time polymerase chain reaction assays and/or D2 ribosomal RNA gene sequencing, as applicable.

Reference Range
Ref. Range Notes
Reference Interval: No growth after 24 days of incubation.

Interpretation:

Positive cultures of yeast and filamentous fungi are reported with the organism identification.

The clinician must determine whether the presence of an organism is significant or not. A final negative report is issued after 24 days of incubation.

Interferences and Limitations

Cautions:

For optimal recovery of organisms, sufficient specimen should be transported within 24 hours of collection.

Fungi can be pathogens, colonizers, or contaminants. Correlation of the patient clinical condition with culture results is necessary.

References

Ordering & Collection

Specimen Type
Bone Marrow
Collection

Collection: Bone marrow in YELLOW SPS (sodium polyanethol sulfonate) tube

  • Also Accepted: Sterile container or GREEN TOP (lithium or sodium heparin) tube.

Expedite transport to the lab.

Handling Instructions

Outside Laboratories:

  • For optimal recovery of organisms, specimens should arrive at Mayo within 24 hours of collection. For this reason, it is recommended that non-local clients arrange for testing directly with Mayo if possible.
  • If sending orders to UW: Refrigerate bone marrow specimen in original collection tube and expedite transport.

Stability: Refrigerated (preferred): 7 days; Ambient: 7 days; Frozen: Unacceptable.

Quantity
Requested: 1 mL bone marrow in SPS tube
Minimum: 1 mL bone marrow in SPS tube

Processing

Receiving Instructions

Refrigerate bone marrow in original collection container.

Log: SPMSND-;REFRIG

  • MSNDTO: MAYO
  • MSPTYP: BNM
  • MTSTRQ: ;Bone Marrow Culture, Fungal (Mayo Test FGEN)

Sendouts:

  • Order Mayo Test: FGEN
  • In MayoAccess, enter the Specimen Source: Bone Marrow. Failure to provide the source at login may result in delays.
  • Expedite transport. Samples are preferred to arrive within 24 hours of collection.

Stability: Refrigerated (preferred): 7 days; Ambient: 7 days; Frozen: Unacceptable.

Misc Sendout

Performance

Lab Department
Frequency
Performed: Monday through Saturday. Reported: 24-35 days from sample receipt at Mayo Clinic Laboratories. Negative culture reported at 24 days.
Available STAT?
No
Performing Location(s)
Sendout Mayo Clinic Laboratories
800-533-1710

200 First Street Southwest
Rochester, MN 55901

Billing & Coding

Billing Comments

CPT: 87102 (Culture only)

  • Reflex testing may be performed at additional charge.