HLA Ab Spec. Monitor DSA (Sendout)

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General Information

Lab Name
HLA Ab Spec. Monitor DSA (Sendout)
Lab Code
RHLAM
Epic Name
HLA Ab Spec. Monitor DSA (Sendout)
External Test Id
3083-17 (Routine), 3083-23 (Protocol), or 3083-18 (STAT), depending on priority requested.
Description

Test Information: The purpose of the test is to monitor patients after transplant for the presence and level of antibodies to the donor’s HLA antigens. The test is semi-quantitative.

Ordering Note: A call-back number must be included for all STAT orders. Enter contact information in the Epic order comments or on the Bloodworks Northwest Requisition.

References
Forms & Requisitions
Synonyms
3083-17, 3083-18, 3083-23, Donor Specific Antibody, DSA, HLA Antibody Specificity-Monitoring DSA – Protocol, Post-Transplant DSA
Components
Code Name
RHLAMR HLA Specific Ab Monitor (Sendout)
S2HLAM HLA Ab Specific Mon. Testing Lab

Interpretation

Method

Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens

Reference Range
See individual components
References

Ordering & Collection

Specimen Type
Blood
Collection

10 mL blood in RED TOP (clot) tube

Unacceptable: GOLD SST

Note: Bloodworks NW does not require dual signatures on the sample labels and requisition for this test.

Forms & Requisitions
Handling Instructions

Outside Laboratories:

  • Transport whole blood samples at room temperature (serum from a red top tube is also accepted). Samples must be received by Bloodworks Northwest within 10 days of collection.
  • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

Stability: Ambient: 10 days.

Quantity
Requested: 7-10 mL blood in red top
Minimum: 2 mL blood in red top

Processing

Receiving Instructions

Store and transport samples at room temperature.

  • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must clearly indicate the Bloodworks NW test to be performed).
  • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag).
    • Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
  • STAT orders should be transported to Bloodworks Northwest as received.
    • Providers may coordinate STAT testing with a Bloodworks Lead Technical Coordinator, who will contact SPS to request expedited transport of a sample. Samples may be sent to Bloodworks 24/7.
  • Send routine samples via scheduled UW DLMP Courier or Delivery Express shipments.
  • UW-MT: Samples are sent by SPS/Cross Hospital.
  • HMC: Store samples in the room temp "H2PSBC" storage bin while awaiting shipment. Samples are sent by HMC Sendouts.

Stability: Ambient: 10 days.

Misc Sendout

Performance

Lab Department
Sendouts(SO)
Frequency
Performed: Monday-Friday. Analytic Time: 2 business days.
Available STAT?
Yes
Performing Location(s)
Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
206-689-6580

921 Terry Ave
6th Floor
Seattle, WA 98104
Other Locations/Notes

Bloodworks Northwest HLA Immunogenetics Lab:

  • Phone: (206) 689-6580
  • Fax: (206) 689-6582
  • Laboratory staffed for questions: Monday through Friday, 07:00 a.m. to 05:00 p.m.
  • After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730.

Billing & Coding

CPT Codes
86834, 86835
LOINC
41618-0
Interfaced Order Code
UOW5461