HLA Antibody Monitoring DSA, Routine (Sendout)

This test replaces HLA Antibody Monitoring DSA, Routine (Sendout) [RHLAM]
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General Information

Lab Name
HLA Antibody Monitoring DSA, Routine (Sendout)
Lab Code
RHLARM
Epic Name
HLA Antibody Monitoring DSA, Routine (Sendout)
External Test Id
3083-17A
Description

Indication: Solid organ transplant.

    Test Information: The purpose of the test is to monitor patients after transplant for the presence and level of antibodies to the donor’s HLA antigens. The test is semi-quantitative.

      Ordering Guidance (For Cause/Routine): Order this test when a problem is suspected with the organ, but STAT results are not needed. Testing is performed Monday through Friday with a 2-day turnaround time. If expedited testing is required, refer to test HLA Antibody Monitoring DSA, STAT (Sendout) [RHLASM]. For protocol DSA testing, see HLA Antibody Monitoring DSA, Protocol (Sendout) [RHLAMP].

      References
      Forms & Requisitions
      Synonyms
      3083-17, 3083-17A, Donor Specific Antibody, DSA, DSA Routine, HLA Antibody Specificity-Monitoring DSA, Post-Transplant DSA, RHLAM
      Components
      Code Name
      RHLAMR HLA Antibody Monitoring DSA Interpretation

      Interpretation

      Method

      Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens

      Reference Range
      See individual components
      References

      Ordering & Collection

      Specimen Type
      Blood
      Collection

      10 mL blood in RED TOP (clot) tube

      • Also Accepted: 6 mL RED TOP (clot) tube

      Unacceptable: GOLD SST

      Forms & Requisitions
      Handling Instructions

      Outside Laboratories:

      • Transport whole blood samples at room temperature (serum from a red top tube is also accepted). Samples must be received by Bloodworks Northwest within 10 days of collection.
      • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

      Stability: Ambient: 10 days. Serum is also accepted.

      Quantity
      Requested: 7-10 mL blood in red top tube
      Minimum: 2 mL blood in red top tube

      Processing

      Receiving Instructions

      Store and transport samples at ambient temperature.

        Sendouts (UW-MT/HMC) and FHCC SPS:

        • Bloodworks NW HLA Test: 3083-17A
        • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group.]
        • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
          • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
        • Send routine samples via scheduled DLMP Courier shipments (or Delivery Express, as needed).

        Stability: Ambient: 10 days. Serum is also accepted.

        Misc Sendout

        Performance

        Lab Department
        Sendouts BWNW Lab (AB)(BWAB)
        Frequency
        Performed: Monday through Friday. Reported: 2 business days from sample receipt at Bloodworks NW HLA Lab.
        Available STAT?
        No. Select test HLA Antibody Monitoring DSA, STAT (Sendout) [RHLASM] if STAT testing is required.
        Performing Location(s)
        Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
        206-689-6580

        921 Terry Ave
        6th Floor
        Seattle, WA 98104

        Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
        After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
        Fax: 206-689-6582

        Billing & Coding

        CPT Codes
        86834, 86835
        LOINC
        41618-0
        Interfaced Order Code
        UOW6051