HLA Antibody Monitoring DSA, Routine (Sendout)

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General Information

Lab Name
HLA Antibody Monitoring DSA, Routine (Sendout)
Lab Code
RHLAM
Epic Name
HLA Ab Spec. Monitor DSA (Sendout)
External Test Id
3083-17A
Description

Indication: Solid organ transplant.

    Test Information: The purpose of the test is to monitor patients after transplant for the presence and level of antibodies to the donor’s HLA antigens. The test is semi-quantitative.

      Ordering Guidance (For Cause/Routine): Order this test when a problem is suspected with the organ, but STAT results are not needed. Testing is performed Monday through Friday with a 2-day turnaround time. If expedited testing is required, refer to test HLA Antibody Monitoring DSA, STAT (Sendout) [RHLASM]. For protocol DSA testing, see HLA Antibody Monitoring DSA, Protocol (Sendout) [RHLAMP].

      References
      Forms & Requisitions
      Synonyms
      3083-17, 3083-17A, Donor Specific Antibody, DSA, HLA Antibody Specificity-Monitoring DSA, Post-Transplant DSA
      Components
      Code Name
      RHLAMR HLA Antibody Monitoring DSA Interpretation

      Interpretation

      Method

      Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens

      Reference Range
      See individual components
      References

      Ordering & Collection

      Specimen Type
      Blood
      Collection

      10 mL blood in RED TOP (clot) tube

      Unacceptable: GOLD SST

      Forms & Requisitions
      Handling Instructions

      Outside Laboratories:

      • Transport whole blood samples at room temperature (serum from a red top tube is also accepted). Samples must be received by Bloodworks Northwest within 10 days of collection.
      • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

      Stability: Ambient: 10 days.

      Quantity
      Requested: 7-10 mL blood in red top
      Minimum: 2 mL blood in red top

      Processing

      Receiving Instructions

      Store and transport samples at ambient temperature.

      Sendouts:

      • Bloodworks NW HLA Test: 3083-17A
      • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group.]
      • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
        • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
      • Send routine samples via scheduled DLMP Courier shipments (or Delivery Express, as needed).

      Stability: Ambient: 10 days. Serum is also accepted.

      Misc Sendout

      Performance

      Lab Department
      Sendouts BWNW Lab (AB)(BWAB)
      Frequency
      Performed: Monday through Friday. Reported: 2 business days from sample receipt at Bloodworks NW HLA Lab.
      Available STAT?
      No. Select test HLA Antibody Monitoring DSA, STAT (Sendout) [RHLASM] if STAT testing is required.
      Performing Location(s)
      Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
      206-689-6580

      921 Terry Ave
      6th Floor
      Seattle, WA 98104

      Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
      After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
      Fax: 206-689-6582

      Billing & Coding

      CPT Codes
      86834, 86835
      LOINC
      41618-0
      Interfaced Order Code
      UOW5461